20.500.12556/RUL-177185 Karakterizacija izbranih acidofilnih in alkalifilnih gliv Characterisation of selected acidophilic and alkaliphilic fungi V magistrski nalogi smo raziskovali zmožnost rasti izbranih acidofilnih in alkalifilnih gliv v širokem razponu pH. S podpornimi eksperimenti smo dodatno preverili esterazno aktivnost in potrebe po kisiku v nalogi vključenih glivnih izolatov. Za namene testiranja rasti gliv v širokem razponu pH smo se ukvarjali tudi z validacijo puferskih sistemov in njihovo obstojnostjo v različnih gojiščih. Z dobljenimi rezultati smo naše hipoteze potrdili, saj so glive, osamljene iz kislega okolja uspevale v gojišču z bazičnimi pH vrednostmi, glive osamljene iz bazičnega okolja pa v gojišču s kislimi pH vrednostmi. Med 11 preučevanimi glivnimi izolati je 10 izolatov uspevalo v širokem razponu pH. To kaže, da glive v širokem razponu pH uspevajo pogosteje, kot smo do sedaj domnevali. Rast v tako široken razponu pH jim omogoča izredna morfološka in fiziološka plastičnost. Pokazali smo tudi, da je večina gliv, osamljenih iz vulkanskega jezera z nizkim pH, acidotolerantnih in ne acidofilnih, z optimalnim pH za rast višjim od pH okolja, iz katerega so bile prvotno osamljene. Podobno so tudi domnevno alkalofilni izolati uspevali v širokem razponu pH, dva pa tudi v kislih razmerah. Zaradi opisanih lastnosti rasti so osamljeni izolati potencialno primerni za biotehnološke namene, predvsem v bioremediaciji kislih in bazičnih okolij. Za poznavanje aplikativnosti bi bile potrebne nadaljnje raziskave na nivoju genomov in transkriptomov izbranih izolatov. In this master's thesis, we investigated the growth of selected acidophilic and alkaliphilic fungi across a wide pH range. In addition, we examined oxygen requirements and esterase activity of tested isolates. To test fungal growth across a broad pH range, we also validated buffer systems and evaluated their effectiveness in different culture media. The obtainted results confirmed our hypotheses, as fungi isolated from acidic environments were able to grow in media with an alkaline pH and vice-versa. Among the 11 tested isolates, 10 thrived across a wide pH range, suggesting that fungi capable of surviving in such diverse pH conditions are more common than previously assumed. This adaptability is enabled by their exceptional morphological and physiological plasticity. We demonstrated that most of the fungi primarily isolated from the acidic lake are acidotolerant rather than acidophilic, as the pH with observed optimal growth was higher than that of their native habitat. Similarly, the putatively alkaliphilic isolates also thrived in a wide pH range, and two also in acidic range. Due to their adaptations in wide pH range the studied isolates have potential applications in biotechnology, particularly in the bioremediation of acidic and alkaline environments. Further research including genomic and transcriptomic analyses of these isolates would be necessary to explore their full biotechnological potential. ekstremofili acidofilne glive alkalifilne glive pH morfologija extremophiles acidophilic fungi alkaliphilic fungi pH morphology true false false [M. Franko] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-12-17 11:34:52 2025-12-17 11:34:56 2025-12-28 04:11:53 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.2:582.28 277573 261954563 55573.pdf 55573.pdf 1 9DE26CD760D777FDFC1828BAEC0D22CD 78c62cd17ff0dc7846e6f1fa8483b286a8ba3435685c5da55dace6c7ddf687b9 96cbb090-db33-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=224084 Biotehniška fakulteta 0 0 0