20.500.12556/RUL-17711 Vsebnost skatola v maščobnem tkivu merjascev določena s spektrofotometrično metodo Zaradi težnje po prepovedi kirurške kastracije merjascev, se razmišlja o alternativah, ki vključujejo tudi pitanje merjascev za prirejo mesa. Problem pri porabi merjaščevega mesa predstavlja predvsem neprijeten vonj, za katerega je v veliki meri odgovoren skatol (3-metilindol). V raziskavi smo vpeljali spektrofotometrično metodo ya določanje vsebnosti skatola v maščobnem tkivu. Zbrali smo vzorce petih genotipv merjascev iz petih različnih rej, starih med 101 in 310 dni. Ob vpeljavi metode smo analizirali prvo skupino, kasneje dobljene vzorce pa kot drugo skupino. Vpeljana metoda je ponovljiva (koeficient variabilnosti, KV = 13,6), obnovljiva (KV = 21,3) in ima dober izkoristek (96 %). Vsebnost skatola v hrbtnem maščobnem tkivu merjascev pri prvi skupini je bila mes 0,01 in 0,62 ppm, v povprečju 0,23 ppm.Druga skupina pa je v povprečju vsebovala 0,71 ppm skatola (med 0,07 in 1,26 ppm). Vpliva genotipa, rejca in starosti na vsebnost skatola nismo zaznali. Due to tendency for banning surgical castration of boars, alternatives such as fattening of boars for human consumption are being considered. Primary problem in boar`s meat consumption is boar`s taint, which is to a large extent the result of skatole (3-methyl indole) content. Spectrophotometric method for determination of skatole in fat tissue was introduced. Samples from three genotypes of boars from five different breedings, aged between 101 and 310 days were collected. When introducing the method, the first group of samples was analyzed, the samples that were obtained later represented a second group. Introduced method is repeatable (coefficient of variability, CV = 13.6), reproducible (CV = 21.3) and has good recovery (96%). Skatole contentin boarʼs back fat in first group was between 0.01 to 0.62 ppm, on average 0.23 ppm. The second group contained on average 0.71 ppm skatol (between 0.07 and 1.26 ppm). Effect of genotype, breeder and age on skatole content was not found. prašiči merjasci hrbtno maščobno tkivo skatol spektrofometrična metoda pigs boars back fat spectrofotometric method true false false Slovenski jezik Angleški jezik Delo ni kategorizirano 2014-07-11 13:54:50 2014-07-11 13:54:50 2022-05-20 03:44:58 0000-00-00 00:00:00 2012 0 0 Str. 37-45 št. 1 Letn. 100 2012 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.4:637.5 1581-9175 3082632 http://aas.bf.uni-lj.si/zootehnika/100-2012/PDF/100-2012-1-37-45.pdf 1 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=17711 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0