20.500.12556/RUL-176277 Odstranjevanje sulfonamidnega antibiotika iz sintetične odpadne vode z algo Chlorella vulgaris in fotokatalizo s titanovim dioksidom magistrsko delo Removal of sulphonamide antibiotic from synthetic wastewater by Chlorella vulgaris and photocatalysis with titanium dioxide master thesis Sulfonamidni antibiotiki so zaradi svoje obstojnosti in uporabe v medicini in veterini pogosta onesnaževala vodnega okolja, ki jih klasični postopki čiščenja odpadnih vod ne odstranijo dovolj učinkovito. Zato se raziskujejo nove, tudi bolj trajnostne metode odstranjevanja farmacevtskih onesnaževal. Preučvali smo sulfisoksazol (SSX), ki se zaradi svoje stabilnosti in širokega spektra delovanja pogosto uporablja pri zdravljenju okužb sečil, dihal in ušes. Namen: Namen raziskave je bil preučiti učinkovitost odstranjevanja sulfisoksazola iz sintetične odpadne vode s sklopljeno tehniko biološkega postopka z algo Chlorella vulgaris in fotokatalize s titanovim dioksidom (TiO2). Posebno pozornost smo namenili primerjavi različnih zaporedij obeh metod (najprej čiščenje z algami nato s fotokatalizo in obratno). Metode dela: V laboratorijskih pogojih smo izvedli poskuse, kjer smo primerjali različne skupine vzorcev v sintetični odpadni vodi z dodanim antibiotikom. Izpostavili smo jih čiščenju z algo C. vulgaris ter fotokatalizi z uporabo TiO2 pod UVA-svetlobo. Spremljali smo razmnoževanje alg, spremembe koncentracije SSX z analizo s HPLC ter osnovne fizikalno-kemijske parametre (temperatura, pH, elektroprevodnost, raztopljeni kisik). Rezultate smo statistično obdelali in primerjali učinkovitost posameznih pristopov ter njihovega vrstnega reda. Rezultati: Razmnoževanja alg ni bilo zavrto ob prisotnosti SSX. V nekaterih skupinah vzorcev je bilo razmnoževanje celo boljše kot v kontrolnih skupinah brez prisotnosti antibiotika. Razgradnja SSX z monokulturo alg ni bila učinkovita, medtem ko smo s fotokatalizo dosegli do 64 % znižanje koncentracije antibiotika. Večjo učinkovitost čiščenja je pokazala kombinacija najprej fotokatalize in nato algnega sistema, kjer smo dosegli 76 % zmanjšanje SSX. Potek razgradnje SSX je bil v tem primeru bolj stalen skozi celotno obdelavo. Razprava in zaključek: Rezultati potrjujejo, da sklopljena uporaba fotokatalize in algnega sistema ponuja sinergijske učinke pri odstranjevanju farmacevtskih onesnaževal kot je npr. SSX. Zaporedje postopkov je ključno, saj je kombinacija najprej fotokatalize in nato algnega sistema zagotovila višjo učinkovitost. Raziskava tako odpira možnosti za razvoj trajnostnih tehnologij čiščenja odpadnih vod, ki vsebujejo sulfonamidne antibiotike, s sklopljenimi tehnikami. Introduction: Sulphonamide antibiotics are common pollutants in aquatic environments due to their persistence and widespread use in human and veterinary medicine. Conventional wastewater treatment processes do not remove them efficiently. Therefore, new sustainable methods for eliminating pharmaceutical contaminants are being investigated. We studied sulfisoxazole (SSX), which is often used for treating urinary tract, respiratory, and ear infections because of its stability and broad-spectrum activity. Objective: The aim of the study was to investigate the efficiency of removing sulfisoxazole from synthetic wastewater using a combined technique of biological treatment with the alga Chlorella vulgaris and photocatalysis with titanium dioxide (TiO₂). Special attention was given to comparing different sequences of the two methods (first algae then photocatalysis, and vice versa). Methods: In laboratory conditions, we conducted experiments comparing different groups of synthetic wastewater samples with added antibiotic. The samples were subjected to treatment with C. vulgaris and photocatalytic treatment using TiO₂ under UVA light. We monitored algal growth, changes in SSX concentration using HPLC analysis, and basic physico-chemical parameters (temperature, pH, conductivity, dissolved oxygen). The results were statistically processed and the efficiency of individual approaches and their sequences was compared. Results: Algal growth was not inhibited by the presence of SSX; in some groups, it was even better than in control samples without the antibiotic. Independent algal degradation of SSX was not effective, whereas photocatalysis achieved significant reduction in antibiotic concentration (up to 64 %). Higher efficiency was achieved by combining photocatalysis first followed by algal treatment, where the final antibiotic concentration decreased to 24 %. The degradation of SSX was more gradual and consistent throughout the treatment. Discussion and Conclusion: The results confirm that the combined use of photocatalysis and algal treatment provides synergistic effects in the removal of pharmaceutical contaminants such as SSX. The sequence of treatments is crucial, as the combination of photocatalysis followed by algae yielded the best results. This study therefore opens possibilities for the development of sustainable wastewater treatment technologies for removing sulphonamide antibiotics using combined techniques. magistrska dela sanitarno inženirstvo sulfisoksazol sulfonamidni antibiotiki Chlorella vulgaris fotokataliza titanov dioksid odpadne vode onesnaževala čiščenje vode master's theses sanitary engineering sulfisoxazole sulphonamide antibiotics Chlorella vulgaris photocatalysis titanium dioxide wastewater contaminants water treatment true false false [N. Cingl] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-11-26 11:03:05 2025-11-26 11:03:12 2025-12-19 04:08:46 0000-00-00 00:00:00 2025 0 Ljubljana 0 Ljubljana 60 str., [2] str. pril. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 614 155119 258770947 57088.pdf 57088.pdf 1 E97EE2DADBB5EA9972B8B242704B8D3E 40ce09e98373a397e5a5805ce1932baddbfdfb7fad188d915b88a173eba0f8d5 c0d71da5-caae-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=222558 Zdravstvena fakulteta 0 0 0