20.500.12556/RUL-176005 Isolation and characterization of Komagataeibacter piraceti sp. nov. and Novacetimonas labruscae sp. nov.: two novel microaerobic cellulose-producing acetic acid bacteria from vinegars The genera Komagataeibacter and Novacetimonas comprise industrially important species that produce various foods, nanocellulose, acetan-like polysaccharides, enantioselective sugars, and other valuable products. Here, we describe two novel strains, Hr1 and Jurk4, isolated from pear and apple-grape organic vinegars that showed very high (≥99.39%) 16S rRNA gene sequence identities to species of the Komagataeibacter and Novacetimonas genera, respectively. However, analysis of the 16S-23S rRNA gene internal transcribed spacer (ITS) sequences revealed only 92.6% sequence identity between the Hr1 strain and its closest relative, Komagataeibacter sucrofermentans LMG 18788T , and 93.8% sequence identity between the Jurk4 strain and its closest relative, Novacetimonas cocois JCM 31140T . Further whole-genome analysis showed for both strains an average nucleotide identity (ANI) below 94% and an in silico DNA–DNA hybridization (dDDH) value of less than 70% to their closest species, supporting their distinction as novel species. The strain Hr1 can be phenotypically differentiated from its closest Komagataeibacter species based on its ability to utilize (NH4)2SO4 as the sole nitrogen source in Asai medium with D-glucose and its inability to grow with 1-propanol as a sole carbon source. The strain Jurk4 can be differentiated from other Novacetimonas type strains based on its ability to produce 5-keto-D-gluconic acid, its growth in a medium with glycerol as the sole carbon source, and its inability to grow in an Asai medium with D-glucose. Both strains produce cellulose and possess clusters for acetane-like polysaccharide production, although of different types, which makes them industrially relevant. Based on these findings, we propose Komagataeibacter piraceti sp. nov. Hr1T (=ZIM B1167T = LMG 33628T ) and Novacetimonas labruscae sp. nov. Jurk4T (=ZIM B1166T = LMG 33630T ) as two novel members of the acetic acid bacteria group. acetic acid bacteria Acetobacteraceae Komagataeibacter Novacetimonas Komagataeibacter piraceti Novacetimonas labruscae vinegar ocetnokislinske bakterije nove vrste ocetnokislinskih bakterij kis hruškov kis jabolčno-grozdni kis true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-18 08:55:44 2025-11-18 08:55:49 2025-12-16 04:08:46 0000-00-00 00:00:00 2025 0 0 19 str. iss. 2, art. 456 Vol. 13 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.6 2076-2607 10.3390/microorganisms13020456 226735619 karnicnik_microorganisms-13-00456-v2.pdf karnicnik_microorganisms-13-00456-v2.pdf 1 0B05B26FCE0E555120F2F1857271853E aa43faa62340091068b93221a7d83a29e05bb51f75d5e7c310384c1c046528ee cda8bf81-c453-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=222027 https://www.mdpi.com/2076-2607/13/2/456 1 a72855a7-c453-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=222026 Biotehniška fakulteta 0 0 0