20.500.12556/RUL-175850 Adaptive immune cell reactivity to polycaprolactone, alginate, chitosan, and zein nanofibers This study explores how nanofiber chemical composition influences human immune cell responses, a key factor in the body's defense against foreign substances. Nanofiber mats composed of polycaprolactone (PCL), alginate (ALG), chitosan (CS), and zein were incubated with both unstimulated and phytohemagglutinin-L-stimulated peripheral blood mononuclear cells (PBMCs), as well as immature and mature monocyte-derived dendritic cells (MoDCs). We evaluated viability, metabolic activity, and cytokine release of PBMCs while in MoDCs, we assessed surface marker expression, phagocytic capacity, and allogeneic T-cell stimulatory function of MoDCs. Results demonstrate that nanofiber composition significantly influenced immune cell responses. While PCL and ALG nanofibers showed no significant effects on PBMCs, those made of CS, reduced their metabolic activity, warranting caution in their use. In contrast, zein nanofibers increased both metabolic activity and pro-inflammatory cytokine release of PBMCs, suggesting their potential immunogenicity. In MoDCs, PCL and CS nanofibers impaired both their phenotypic maturation and functional performance, including antigen uptake and presentation. Zein nanofibers induced changes in MoDC surface marker expression, however their overall functionality remained intact. In contrast, ALG nanofibers exhibited the highest biocompatibility, with minimal impact on both immature and mature MoDCs. This favorable profile of ALG nanofibers supports their suitability for biomedical applications, such as tissue engineering and drug delivery. Overall, our findings underscore the importance of thorough immunological safety assessment of nanofiber-based biomaterials prior to their clinical use. polymers dendritic cells PBMCs immunity safety chemical composition nanofibers polimeri dendritične celice PBMC imunost varnost kemijska sestava nanovlakna imunski odziv true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-11 09:10:12 2025-11-11 09:10:18 2026-06-18 09:35:53 0000-00-00 00:00:00 2025 0 0 10 str. pt. 2, art. 147562 Vol. 328 Nov. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2616-097 0141-8130 10.1016/j.ijbiomac.2025.147562 250776067 1-s2.0-S014181302508119X-main.pdf 1-s2.0-S014181302508119X-main.pdf 1 12A34369BA56386BC5E5D3A44C911DA9 e5e0bfb5ced5e0044776821e501515335aa39346ecd3924d93cd6cb682c1a96e 14359fd2-bed6-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221754 https://www.sciencedirect.com/science/article/pii/S014181302508119X 1 882c5a6f-bed5-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221752 Fakulteta za farmacijo 0 0 0