20.500.12556/RUL-175790 One-pot HPLC method for simultaneous quantification of hydrogen peroxide and organic peroxides Hydrogen peroxide and organic peroxides are widely used in the pharmaceutical and food industries as disinfectants, preservatives, and bleaching agents. They can also form as by-products during polymerization, drying, or degradation processes, potentially compromising product quality. While current analytical methods typically measure total peroxide content, they do not distinguish between hydrogen peroxide and organic peroxides, despite their differing reactivity and implications for product stability. We developed a one-pot HPLC method for the simultaneous quantification of hydrogen peroxide and organic peroxides. The method utilizes borinic acid for the selective reaction with hydrogen peroxide, followed by the reaction of triphenylphosphine with the remaining organic peroxides. The resulting phenol derivative and triphenylphosphine oxide are detected using HPLC with UV detection. The method demonstrated strong performance across a variety of complex matrices, including pharmaceutical excipients, milk, honey, and vegetable oil. Quantification limits were 4.4 nmol/g for hydrogen peroxide and 9 nmol/g for organic peroxides. The method's practical utility was demonstrated using famotidine, a pharmaceutical compound selectively oxidized by hydrogen peroxide but not by organic peroxides. This application highlights the method's ability to guide excipient selection based on specific peroxide profiles and to support antioxidant use in formulation development. By enabling the differentiation of peroxide species, this approach facilitates the development of more stable and cost-effective formulations. This novel HPLC method offers a simple, robust, and versatile tool for the selective quantification of hydrogen peroxide and organic peroxides in a wide range of applications, including pharmaceutical and food analysis, where precise peroxide monitoring is essential. peroxide quantification hydrogen peroxide organic peroxides borinic acid liquid chromatography HPLC kvantifikacija peroksida vodikov peroksid organski peroksidi borova kislina tekočinska kromatografija HPLC analizna kemija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-07 16:10:16 2025-11-07 16:10:22 2025-12-15 03:52:08 0000-00-00 00:00:00 2025 0 0 8 str. art. 114889 Vol. 217 Oct. 2015 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.426 1095-9149 10.1016/j.microc.2025.114889 255033859 One-pot-HPLC-method-for-simultaneous-quantification-of-hyd_2025_Microchemica.pdf One-pot-HPLC-method-for-simultaneous-quantification-of-hyd_2025_Microchemica.pdf 1 8B56A62BEDBB19F7720B1EB9FBA1DFD0 21b270968f0eb466c3ddc688432785b795daa48fa60ff2c3edc2daf5a07172e7 550da8c0-bbed-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221662 https://www.sciencedirect.com/science/article/pii/S0026265X25022374 0 8ffa1d99-bbeb-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221660 Fakulteta za farmacijo 0 0 0