20.500.12556/RUL-175776 Optimizing germination protocols for Citrullus colocynthis a study on seed dormancy breakthrough through chemical and physical treatments Optimiziranje protokolov kalitve kolokinte (Citrullus colocynthis (L.) Schrad.): Študija o prekinitvi mirovanja semen s kemičnimi in fizikalnimi obdelavami Bitter apple (Citrullus colocynthis), a desert plant with medicinal properties, typically exhibits low seed germination due to dormancy. This study investigated physical and chemical treatments to improve C. colocynthis seed germination. A completely randomized design with factorial arrangement and three replications was used to test the effects of gibberellic acid (GA3) at concentrations of 0, 10, 50, 100, and 150 ppm, under four diurnal temperature and light (TL) regimes: (1) 20°C (12h light/dark), (2) 25°C (12h light/dark), (3) 35°C (12h light) / 15°C (12h dark), and (4) 30°C (12h light) / 20°C (12h dark). Results showed that TL regime, GA3, and their interaction significantly affected all measured germination parameters. The highest germination percentage, relative germination percentage, mean daily germination, germination index, germination value, and seedling dry mass were achieved with 100 ppm GA3 under the 35-15°C regime. The study concluded that average germination speed, germination time variation coefficient, germination speed coefficient, and synchronization index were unreliable for assessing seed quality under low germination conditions, suggesting that physiological dormancy in Bitter Apple seeds may stem from embryo immaturity, inhibitory factors, or both. seed dormancy physiological dormancy diurnal temperature alternation gibberellic acid dormanca semen fiziološka dormanca diurnalno menjavanje temperatur giberilinska kislina true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-07 10:12:55 2025-11-07 10:13:03 2025-12-15 03:49:23 0000-00-00 00:00:00 2025 0 0 11 str. no. 3 Vol. 121 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 1854-1941 10.14720/aas.2025.121.3.22753 256318467 View_of_Optimizing_Germination_Protocols_for_Citrullus_colocynthis__A_Study_on_Seed_Dormancy_Breakthrough_through_Chemical_and_Physical_Treatments.pdf View_of_Optimizing_Germination_Protocols_for_Citrullus_colocynthis__A_Study_on_Seed_Dormancy_Breakthrough_through_Chemical_and_Physical_Treatments.pdf 1 84D9CBE9195DE56A889F622DEA8F1430 8fc54521509f83efa8c4c73a3e50938ac837316a5bdc4c6f176389cd74d6d108 d76fb7e4-bbb9-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221629 https://journals.uni-lj.si/aas/article/view/22753/19019 1 a5286eef-bbb9-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221628 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0