20.500.12556/RUL-175625 Influence of vegetable oils and their constituents on in vitro human keratinocyte and fibroblast proliferation and migration Human skin is a complex organ essential for protection, hydration and regeneration, with keratinocytes and fibroblasts playing pivotal roles in wound healing and tissue renewal. This study investigates the effects of selected vegetable oils, fatty acids and unsaponifiable compounds on the proliferation and migration of human keratinocytes and fibroblasts in vitro. Oils were selected based on their specific fatty acid profiles, including coconut, olive, linden, poppy, pomegranate, marigold and linseed oils. Using cell proliferation and gap closure assays, the impact of these oils and their constituents on cell proliferation and migration was evaluated. Results showed that the tested biological activity of the oils depended primarily on the fatty acid composition of their triglycerides. Oils rich in essential fatty acids, particularly linoleic acid, significantly promoted cell proliferation. In contrast, unsaponifiable compounds showed no effect, while punicic acid and pomegranate seed oil had a significant negative impact. The oils did not exert notable effects on the closure rate of the artificial wound gap; however, sterculic and oleic acids inhibited gap closure and induced changes in cell morphology. These findings support the role of plant oils and their composition in enhancing skin cell regeneration, and provide in vitro evidence to support their application in cosmetic and dermatological formulations aimed at skin repair and evidence-based skincare. fatty acids fibroblasts keratinocytes proliferation unsaponifiable compounds vegetable oils wound healing fibroblasti keratinociti proliferacija neumiljive spojine celjenje ran maščobne kisline rastlinska olja true false true Angleški jezik Slovenski jezik Članek v reviji 2025-11-06 10:53:24 2025-11-06 10:53:30 2025-12-13 03:52:36 0000-00-00 00:00:00 2025 0 0 17 str. article no. ǂ26898 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 547.466.3+664.34 2045-2322 10.1038/s41598-025-09711-7 250738179 s41598-025-09711-7.pdf s41598-025-09711-7.pdf 1 AC869C2A57C925F8D955642A604962B0 cfd5a23de5a4008a1d60156dac06b3af6dc45a19a850e9afc1325dbe1690215c 8edc9f5d-baf6-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221454 https://www.nature.com/articles/s41598-025-09711-7 1 22e9f4fc-baf6-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=221453 Fakulteta za farmacijo 0 0 0