20.500.12556/RUL-175417 Predklinično testiranje varnosti in imunoreaktivnosti implantata iz bakrove zlitine na mišjem modelu magistrsko delo Preclinical testing of the safety and immunoreactivity of a copper alloy implant in a mouse model M. Sc. thesis Okužbe ortopedskih in zobnih implantatov so kljub napredku medicine še vedno pogost zaplet, ki ga povzroča predvsem tvorba bakterijskih biofilmov in s tem zmanjšana učinkovitost imunskega sistema ter antibiotikov. Eden od pristopov za zmanjšanje tega tveganja je uporaba implantatov z antibakterijskimi lastnostmi. V naši raziskavi smo preučevali titanovo zlitino z dodatkom 4 % bakra (Ti-6Al-4V-4Cu) na mišjem modelu C57BL/6JOlaHsd. Naš cilj je bil oceniti morebitne sistemske učinke implantata na rast, metabolizem in splošno počutje živali. Spremljali smo telesno maso, zauživanje krme in vode ter vedenjske odzive. Ugotavljali smo tudi vpliv implantata na hematološke in biokemijske parametre kot pokazatelje imunskega odziva in delovanja organov. Rezultati so pokazali, da dodatek bakra v zlitino ni povzročil sistemskega vnetja ali toksičnosti, saj so vrednosti krvnih parametrov ostale v referenčnih mejah, prav tako ni bilo razlik v prirastu in zauživanju krme ter vode med skupinami. Naše ugotovitve potrjujejo, da je zlitina Ti-6Al-4V-4Cu biokompatibilna in predstavlja obetaven material za nadaljnje raziskave, kjer bi lahko baker zaradi svojih protibakterijskih lastnosti zmanjšal pojavnost okužb po vsaditvi. Despite advances in medicine, infections of orthopedic and dental implants are still a common complication, caused primarily by the formation of bacterial biofilms, which reduces the effectiveness of the immune system and antibiotics. One approach to reduce this risk is to use implants with antibacterial properties. In our study, we investigated a titanium alloy with 4% copper (Ti-6Al-4V-4Cu) in a C57BL/6JOlaHsd mouse model. Our goal was to evaluate potential systemic effects of the implant on the growth, metabolism, and overall well-being of the animals. We monitored body weight, feed and water intake, and behavioral responses. We also determined the effect of the implant on hematological and biochemical parameters as indicators of immune response and organ function. The results showed that incorporation of copper into the alloy did not induce systemic inflammation or toxicity, as blood parameter values remained within reference ranges, and no differences were observed in weight gain or feed and water consumption between groups. These findings confirm that the Ti-6Al-4V-4Cu alloy is biocompatible and potentially useful for further research, where the additional antibacterial properties of copper could improve the safety of implants. mišji modeli bakrova zlitina biokompatibilnost imunoreaktivnost hematološki parametri biokemijski parametri ortopedski implantati mouse models copper alloy biocompatibility immunoreactivity hematological parameters biochemical parameters orthopedic implants true false false A. Brunčić Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-10-26 07:15:16 2025-10-26 07:15:28 2025-12-10 03:52:29 0000-00-00 00:00:00 2025 0 Ljubljana 2025 Ljubljana 1 spletni vir (1 datoteka PDF (XI, [50] str.)) 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 636.028(043.2) 267580 255908611 54613.pdf 54613.pdf 1 481DC2A0E17750777352D69BE69248EB 66c1fed489e295a71ed416e10c5ca9fa9166a301896b75e44388b6234b9191cf e9232e38-b232-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220948 Biotehniška fakulteta 0 0 0