20.500.12556/RUL-175388 Prevalence and natural impact of major wheat viruses in Azerbaijan Razširjenost in naravni vplivi pomembnejših virusov pšenice v Azerbajdžanu Cereal viruses such as Wheat streak mosaic virus (WSMV), Barley/Cereal yellow dwarf viruses (B/CYDV), and Wheat dwarf virus (WDV) often occur alongside other wheat pathogens, making it difficult to diagnose and manage these diseases effectively. To better understand the current situation with these important DNA and RNA viruses, we collected 157 wheat samples showing virus-like symptoms during the 2022–2024 growing seasons. The samples were taken from wheat-growing areas of Azerbaijan, including Absheron, Jalilabad, and Shamakhi, and were analyzed using ELISA and RT-PCR. During our field surveys, we observed potensial signs of viral infections, such as stunted growth, yellowing or streaking of leaves, and reduced grain yields. After extracting total RNA and DNA from the samples, we used specific primers to amplify regions of the viruses’ genomes. We successfully amplified DNA fragments of 404 bp, 178 bp, and 550 bp in 25, 39, and 44 samples infected with BYDV WSMV, and WDV respectively. These findings shed light on the prevalence of these viruses in Azerbaijan’s wheat fields and provide essential information for improving management strategies to protect wheat productivity. cereal viruses survey detection ELISA RT-PCR WSMV BYDV WDV virusi žit pregled odkrivanje RT-PCR WSMV BYDV WDV true false true Angleški jezik Slovenski jezik Članek v reviji 2025-10-24 15:13:05 2025-10-24 15:13:10 2025-12-10 03:47:07 0000-00-00 00:00:00 2025 0 0 8 str. no. 3 Vol. 121 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 1854-1941 10.14720/aas.2025.121.3.21670 254729475 https://journals.uni-lj.si/aas/article/view/21670/19018 1 e634dac8-b0da-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220916 View_of_Prevalence_and_natural_impact_of_major_wheat_viruses_in_Azerbaijan.pdf View_of_Prevalence_and_natural_impact_of_major_wheat_viruses_in_Azerbaijan.pdf 1 305BDD7F54EA274C2475DFCE09D8BD4D 1c7d8bbeb810675262ef85fba6c23daddb4ee4d5dfa94836e64a3391c6f5df04 887f9f79-b0db-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220917 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0