20.500.12556/RUL-175310 Mikrobiom korenin in akumulacija kovin pri navadni barvilnici (Phytolacca americana) v Sloveniji Root microbiome and metal accumulation in the American pokeweed (Phytolacca americana) in Slovenia Navadna barvilnica (Phytolacca americana) je invazivna tujerodna rastlina, ki se v Sloveniji pojavlja na različnih ruderalnih rastiščih in potencialno spreminja abiotske lastnosti prsti ter sestavo združbe mikrobioma. Cilj magistrske naloge je bil kvantitativno oceniti vpliv vrste P. americana na sestavo združb bakterij, arhej in gliv v prsti treh sestojev v bližini gradu Jable (Trzin). Z metagenomskim sekvenciranjem vzorčnih mest pod barvilnico, pol metra izven sestoja ter en meter izven sestoja smo potrdili, da obstajajo statistično značilne razlike v sestavi relativnih abundanc bakterijskih debel Pseudomonadota, Bacteroidota, Bacillota in Candidatus Colwellbacteria. Rezultati analize taksonomske sestave bakterijskih združb nakazujejo, da prisotnost vrste P. americana spremeni združbo bakterij v smeri spremembe metabolizma N. Poskušali smo opredeliti tudi mehanizem, s katerim vrsta P. americana vpliva na sestavo združbe mikrobioma prsti. Na podlagi literature smo predvidevali, da gre za acidifikacijo prsti, ki poveča dostopnost P in drugih nutrientov v prsti ter v nekaterih primerih vodi v hiperakumulacijo Mn in Al. Zaradi kompleksnosti sistema in omejenega števila vzorcev mehanizma nismo uspeli potrditi. Ugotovili pa smo, da je preiskovana populacija vrste P. americana hiperakumulator Al, medtem ko hiperakumulacija Mn ni bila potrjena. The American pokeweed (Phytolacca americana) is an invasive alien plant species that occurs in various ruderal habitats in Slovenia and potentially alters the abiotic properties of soil as well as the composition of microbial communities. The aim of this master’s thesis was to quantitatively assess the impact of P. americana on the composition of bacterial, archaeal, and fungal communities in soils of three stands near Grad Jable (Trzin). Metagenomic sequencing of samples collected beneath P. americana plants, 0.5 m outside the stands, and 1 m outside the stands revealed statistically significant differences in the relative abundances of the bacterial phyla Pseudomonadota, Bacteroidota, Bacillota, and Candidatus Colwellbacteria. Taxonomic analyses further indicated that the presence of P. americana shifts bacterial community composition towards changes in nitrogen metabolism. We also sought to identify the mechanism by which P. americana influences soil microbiome composition. Based on previous studies, we hypothesized that soil acidification increases the availability of phosphorus and other nutrients, and in some cases leads to the hyperaccumulation of Mn and Al. Due to the complexity of the system and the limited number of samples, the mechanism could not be confirmed. However, we established that the studied population of P. americana is a hyperaccumulator of Al, while hyperaccumulation of Mn was not confirmed. mikrobiom prsti bakterije arheje glive invazivne rastline Phytolacca americana Mn Al soil microbiome bacteria archaea fungi invasive plants Phytolacca americana Mn Al true false false [E. Gregorc] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-10-24 07:16:01 2025-10-24 07:16:07 2025-12-09 04:01:25 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 631.41:631.811(043.2) 267560 254791939 54372.pdf 54372.pdf 1 D3D9BBFC741E4B223D04F0164E7AEF99 93c1b1f226b864e87fdefafe18737ecc467642a76ddbdc9d579023d578c14934 4125c3c4-b098-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220757 Biotehniška fakulteta 0 0 0