20.500.12556/RUL-175309 Morfološka in imunohistokemična analiza telocitov sečnega mehurja miši Morphological and immunohistochemical analysis of telocytes in mouse urinary bladder Telociti (TC) so vrsta intersticijskih celic z značilnimi, dolgimi izrastki, imenovanimi telopodi. Namen magistrske naloge je bil najti specifične molekulske označevalce za TC v sečnem mehurju miši, s katerimi bi lahko opredelili njihov imunofenotip. Analizirali smo tudi ultrastrukturo TC (s TEM), njihovo prostorsko razporeditev v lamini propriji (s svetlobnim mikroskopom na poltankih in parafinskih rezinah) in proliferacijsko aktivnost (z imunooznačevanjem proteina Ki-67), pri čemer smo za vse štiri značilnosti TC iskali tudi razlike v normalnem in kronično vnetem sečnem mehurju. Opazili smo, da so v normalnem sečnem mehurju TC organizirani v tanki plasti, vzporedni s površino urotelija, v kronično vnetem mehurju pa so neurejeno orientirani v vse smeri. TEM analiza je razkrila ultrastrukturne razlike TC normalnega in kronično vnetega sečnega mehurja v izgledu telopodov ter prisotnosti zunajceličnih in znotrajceličnih veziklov. Z imunofluorescenčnim in imunoelektronskim označevanjem smo potrdili, da so CD34, Cx43, eNOS, ENTPD2, PDGFRβ in P2X4 specifični molekulski označevalci TC v sečnem mehurju miši. Z dvojnimi imunooznačevanji teh označevalcev smo razločili tri subpopulacije TC v normalnem sečnem mehurju miši. Posamezne subpopulacije TC se med seboj ločijo v izražanju eNOS, CD34 in P2X4, vse tri pa izražajo Cx43, ENTPD2 in PDGFRβ. Ugotovili smo, da je proliferacijska aktivnost TC v kronično vnetem sečnem mehurju intenzivnejša kot v normalnem sečnem mehurju. Telocytes (TCs) are a type of interstitial cell with specific, long cytoplasmic extensions, called the telopods. This master's thesis aimed to find specific molecular markers of telocytes in the mouse urinary bladder to determine their immunophenotype. We also analysed the ultrastructure of TC (using TEM), their spatial orientation in the lamina propria (using light microscopy), and their proliferative activity (by Ki-67 immunofluorescence labelling). In all these cases, we also sought to identify potential differences between the normal and the chronically inflamed mouse urinary bladders. In a normal urinary bladder, TCs are arranged in thin layers parallel to the urothelial axis, whereas in the chronically inflamed bladder, they are disorganized and oriented in different directions. Regarding TEM analysis of the ultrastructure of TCs, we observed differences between normal and inflamed bladders in terms of telopode shape and the amount of intracellular and extracellular vesicles. Using immunofluorescent and immunogold labelling, we determined CD34, Cx43, eNOS, ENTPD2, PDGFRβ, and P2X4 as specific molecular markers of TCs. Based on double immunolabelling of these markers, we distinguished three subpopulations of TCs in the normal urinary bladder, which show differential expression of eNOS, CD34, and P2X4, while all three express Cx43, ENTPD2, and PDGFRβ. The proliferative activity was more intense in TCs of the chronically inflamed than in the normal mouse urinary bladders. telociti sečni mehur imunohistokemija morfologija mikroskopija telocytes urinary bladder immunohistochemistry morphology microscopy true false false [M. Jernejc] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-10-24 07:15:55 2025-10-24 07:15:59 2025-12-09 04:01:10 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 576.31:616.62-076(043.2) 267559 254792963 54629.pdf 54629.pdf 1 FC58C684133B1607FB5A9211D109B6BB 9edc77bf614403a8036b26ad9dfa0ca2e019c951e07ee796bbed84ff0651c05c 3d000467-b098-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220756 Biotehniška fakulteta 0 0 0