20.500.12556/RUL-175182 Successful micropropagation of critically endangered Thymus bovei Benth a wild medicinal plant strictly endemic to the Jordanian environment Uspešna razmnožitev kritično ogrožene vrste materine dušice (Thymus bovei Benth), samonikle zdravilne rastline iz Jordanije Thymus bovei Benth. (Lamiaceae), locally referred to as Zateer Barry, is a critically endangered native plant species in Jordan (Taifour and EL-Oqlah, 2014), renowned for its exceptional medicinal properties. This study aims to establish a reliable micropropagation technique to prevent T. boveifrom extinction. The research focused on explant establishment, shoot multiplication, rooting, callus induction, and acclimatization of T. bovei. Explants were established in a half-strength Murashige and Skoog (MS) media supplemented with different concentrations of gibberellic acid (GA3). Successful shoot multiplication was achieved by subculturing nodal segments onto half-strength MS medium supplemented with kinetin (KIN), thidiazuron (TDZ), or benzylaminopurine (BA). Among the treatments, the most effective medium for shoot multiplication was supplemented with 2.5 mg l-1 KIN and 0.5 mg l-1 GA₃, which resulted in an average of 7.50 ± 0.54 micro-shoots per explant and a shoot length of 2.33 ± 0.18 cm. Rooting was most effective on half-strength MS media with 1.0 mg l-1 indole-3-butyric acid (IBA), yielding an average of 5.29 roots per microshoot. The highest callus development, measured by fresh mass (0.601 g), occurred in half-strength MS media with 2.0 mg l-1 2,4-D, while no callus formed with naphthaleneacetic acid (NAA). Acclimatized plants showed a 90 % survival rate when transferred to greenhouse conditions. endangered plant Jordan medicinal plant micropropagation tissue culture Thymus bovei ogrožene rastline Jordanija zdravilne rastline mikropropagacija tkivne kulture Thymus bovei true false true Angleški jezik Slovenski jezik Članek v reviji 2025-10-20 14:53:03 2025-10-20 14:53:09 2025-12-07 03:48:52 0000-00-00 00:00:00 2025 0 0 11 str. no. 3 Vol. 121 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 63 1854-1941 10.14720/aas.2025.121.3.18920 253923843 View_of_Successful_Micropropagation_of_critically_endangered_Thymus_bovei_Benth__a_Wild_Medicinal_Plant_Strictly_Endemic_to_the_Jordanian_Environment.pdf View_of_Successful_Micropropagation_of_critically_endangered_Thymus_bovei_Benth__a_Wild_Medicinal_Plant_Strictly_Endemic_to_the_Jordanian_Environment.pdf 1 67C580CDDC72969CADF21F7B51E9AE3C 91ba15018c687c2bdd1b1bb2d6438cdbd6f7e083c187da5ccbf73c598d5e7f86 bd68678e-adb3-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220496 https://journals.uni-lj.si/aas/article/view/18920/19022 1 7382c52e-adb3-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220495 Biotehniška fakulteta Acta agriculturae Slovenica 0 0 0