20.500.12556/RUL-175130 Bacterial and chemical interactions with iron oxide/hydroxide stalagmites using ATR-IR spectroscopy and its 4th derivative spectra Here we report on the chemical interaction between bacteria and fast-growing iron oxide/hydroxide sulphate stalagmites in the abandoned Sitarjevec mine in Slovenia with a low pH (2.2 to 3.4) environment. SEM micrographs revealed distinct morphologies of dry particles from both water and stalagmite layers, as well as the presence of bacterial cells, EPS residue, and biofilm. Bacterial adhesion was studied on the stalagmite surface and inside the stalagmite. Comparison of ATR-IR spectra and the corresponding 2nd and 4th derivative spectra revealed the presence of the P-OFe, COO$^-$ and SO$_4^{2-}$ modes, which are thought to be responsible for bacterial adhesion to the surface and were detected also in the inner part of the stalagmite. To our knowledge, this is the first study of its kind to confirm persistent chemical interactions between iron oxide/hydroxide sulphate-based stalagmite minerals and bacteria on their surface, which is also maintained in the core of the stalagmite. bacterial adhesion bacteria geochemistry infrared spectroscopy mineralogy X-ray spectroscopy ATR-IR – 2nd and 4th derivative spectra mine water iron oxide/hydroxide sulphate minerals interaction with bacteria SEM adhezija bakterij bakterije geokemija infrardeča spektroskopija mineralogija rentgenska spektroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-10-17 10:45:37 2025-10-17 10:45:43 2026-06-08 11:49:33 0000-00-00 00:00:00 2025 0 0 Objavljen je popravek tega članka (gl. datoteko Priloga – Erratum): Mine water and the environment, 2025, Vol. 44, iss. 4, p. 887, DOI: https://doi.org/10.1007/s10230-025-01075-8. Str. 661–675 iss. 3 Vol. 44 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 550.4 1616-1068 10.1007/s10230-025-01071-y 246645763 RAZ_Kovac_Minka_2025.pdf RAZ_Kovac_Minka_2025.pdf 1 7DC931E562851FF0842793B3577A1EE7 81d648a4797cc52ac720c366804a6cb32e032ba131efeb7149d5ef66cbfd2d2e 9fb44c3d-ab35-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220416 s10230-025-01075-8.pdf s10230-025-01075-8.pdf Erratum Erratum 1 23A58ACD729B55293A7121CB607D3FF2 96cd610525d604fa3d74368225d851560f05a803ad57f8dc7688e8ac0f236be8 a5858d8d-60ed-11f1-b0ab-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235019 https://link.springer.com/article/10.1007/s10230-025-01071-y 0 67197cee-ab35-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220415 Fakulteta za strojništvo 0 0 0