20.500.12556/RUL-175082 Targeting intermolecular interactions to reduce viscosity in monoclonal antibody formulations a review Monoclonal antibodies (mAb) have transformed modern medicine, offering targeted therapies for cancer, autoimmune disorders, and infectious diseases. To enhance patient convenience, subcutaneous administration is increasingly prioritized, requiring highly concentrated formulations. However, high viscosity of these formulations hinders manufacturability, injectability, and stability. Viscosity-reducing additives are a promising solution, disrupting protein-protein interactions and thereby reducing the viscosity of mAb formulations. Most promising additives are small organic electrolytes, where key properties influencing their efficacy include charge, size, and salt form. Relatively small molecules with three or more charges are most efficient, while the counter-ion provides opportunity for increased solubility and additional interactions. Our data analysis further highlights topological polar surface area and heteroatoms as significant predictors of viscosity reduction, while the role of aromatic moieties remains questionable. Another promising strategy is combining viscosity-reducing additives, which can achieve synergistic viscosity reduction while enhancing stability. Polyelectrolytes also demonstrate promising efficacy via complex formation with mAbs. Conversely, other common excipients of mAb formulations, such as sugars and surfactants, often increase viscosity of the formulation. This review provides a foundation for advancing viscosity-reducing strategies and offers actionable insights to guide future research and development of effective, patient-oriented mAb formulations. monoclonal antibody protein-protein interactions biopharmaceuticals highly concentrated mAb formulations viscosity-reducing additives subcutaneous administration monoklonska protitelesa interakcije beljakovina-beljakovina biofarmacevtski izdelki visoko koncentrirane formulacije monoklonskih protiteles dodatki za zmanjšanje viskoznosti subkutana uporaba farmacevtska kemija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-10-15 09:18:27 2025-10-15 09:18:34 2026-06-17 16:24:52 0000-00-00 00:00:00 2025 0 0 14 str. pt. 2, art. 147515 Vol. 327 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-097 0141-8130 10.1016/j.ijbiomac.2025.147515 248572419 1-s2.0-S0141813025080729-main.pdf 1-s2.0-S0141813025080729-main.pdf 1 3727F244AE21A88AA92C9A410AEA25F0 dc43951927146fe849d0aafe374b1ef0a026c562683bdeb16fab3b195fd90df8 a03898bc-a997-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220122 https://www.sciencedirect.com/science/article/pii/S0141813025080729 1 e90f8230-a996-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=220121 Fakulteta za farmacijo 0 0 0