20.500.12556/RUL-174611 Bacteriophage T4 propagation in E.coli exposed to severe substrate limitation Understanding bacteriophage propagation on bacteria in different physiological conditions is imperative for predicting phage therapy efficacy on various bacterial infections, especially chronic ones. We investigated phage T4 propagation on bacteria E.coli grown in a chemostat at very low dilution rates extending down to 0.027 h$^{−1}$ and bacteria exposed to nutrient deprivation. An increase in adsorption constant and latent period with dilution rate D decrease and burst size being proportional to dilution rate (D) was confirmed, consistent with previously published results, extending validity of previous findings. Additional bacterial exposure to starvation, either through nutrient cessation or transferring bacteria into SM buffer, sustained phage propagation during first hours of starvation and diminished to formation of a single phage per infected cell after 24 h. Nutrient deprivation effects were investigated on fast growing bacteria and bacteria in a death phase. While no phage generation was observed within bacteria in death phase, fast growing bacteria transferred into SM buffer generated a single phage within 48 h without lysis, indicating that bacterial exposure to nutrient depleted conditions triggers a so called “scavenger response” whose intensity depends on starvation exposure time. bacteriophage T4 bacteriophage growth parameters chemostat E. coli scavenger response substrate limitation bakteriofag T4 bakteriofagni rastni parametri kemostat E. coli scavenger odziv limitacija hranil true false true Angleški jezik Slovenski jezik Članek v reviji 2025-10-07 09:36:52 2025-10-07 09:36:53 2025-11-24 03:46:57 0000-00-00 00:00:00 2025 0 0 15 str. Vol. 22, art. 304 2025 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.3:578.347 1743-422X 10.1186/s12985-025-02934-0 251595523 RAZ_Lisac_Ana_2025.pdf RAZ_Lisac_Ana_2025.pdf 1 FF3E7A87E84B41BD6F710A3E71F51824 4ba5a4d509892101f2952d68e1c5f4bf84b890ced9ad0d0b2b065a48a3ed5982 03fa2431-a351-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=219453 https://virologyj.biomedcentral.com/articles/10.1186/s12985-025-02934-0 1 2a442b22-a350-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=219452 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0