20.500.12556/RUL-174517 Piezoelektrični biomateriali na osnovi poli-L-laktida, modificirani z delci cink-oksida Piezoelectric biomaterials based on poly-L-lactic acid modified with ZnO particles Regenerativna medicina se sooča z izzivom razvoja naprednih biomaterialov, ki ne le podpirajo rast celic, temveč hkrati aktivno sodelujejo pri regeneraciji tkiva. Klasični polimerni materiali pogosto ne zagotavljajo zadostne bioaktivnosti, zaradi česar narašča interes za funkcionalizacijo teh materialov s piezoelektričnimi in tudi bioaktivnimi dodatki. Zanimanje za piezoelektrične materiale izhaja iz dejstva, da lahko kot odgovor na mehanske dražljaje proizvajajo električni naboj, kar spodbuja celične procese, pomembne za celjenje tkiv. Kljub temu potencialu pa ostaja izziv, kako doseči optimalne mehanske, površinske in biološke lastnosti takšnih materialov, ne da bi ogrozili njihovo biokompatibilnost. Namen magistrskega dela je bila priprava inovativnih piezoelektričnih biomaterialov na osnovi poli-L-laktida (PLLA) z dodatkom nanodelcev ZnO za uporabo v regenerativni medicini. Glavni cilji so vključevali optimizacijo strukturnih in površinskih lastnosti ter oceno biološke učinkovitosti materialov. Strukturna analiza je pokazala, da dodatek ZnO ni izboljšal kristaliničnosti ali orientiranosti verig PLLA v takšni meri, kot smo pričakovali. Čeprav sta to ključna parametra za piezoelektrični odziv, se je piezoelektričnost filmov občutno povečala, predvsem zaradi prispevka nanodelcev ZnO, ki so s svojo lastno piezoelektričnostjo in vplivom na strižno deformacijo izboljšali celokupni odziv. Površinske lastnosti so bile uspešno optimizirane. Dodatek ZnO in plazemska obdelava sta povečala hidrofilnost ter hrapavost površine in omogočila večjo izpostavljenost nanodelcev. To je izboljšalo adhezijo človeških celic. Hkrati smo dosegli celično proliferacijo po piezostimulaciji z ultrazvokom. Pri testiranju piezostimulacije so PLLA/ZnO filmi pokazali antimikrobni učinek, ki je bil najizrazitejši pri višjih koncentracijah ZnO, vendar so te bile hkrati tudi citotoksične. Optimalno razmerje med biokompatibilnostjo in protimikrobnostjo je bilo doseženo pri koncentraciji 1 wt% (utežni odstotek) ZnO, kjer so bili rezultati za rast človeških celic in zaviranje bakterij najugodnejši. Regenerative medicine faces the challenge of developing advanced biomaterials that not only support cell growth but also actively participate in tissue regeneration. Conventional polymeric materials often do not provide sufficient bioactivity, which is why there is a growing interest in the functionalization of these materials with piezoelectric and also bioactive additives. The interest in piezoelectric materials arises from the fact that they can generate an electric charge in response to mechanical stimuli, which promotes cellular processes important for tissue healing. Despite their potential, the challenge remains how to achieve optimal mechanical, surface, and biological properties of such materials without compromising their biocompatibility. The aim of the master’s thesis was the preparation of innovative piezoelectric biomaterials based on poly-L-lactide (PLLA) with the addition of ZnO nanoparticles for application in regenerative medicine. The main objectives included the optimization of structural and surface properties and the evaluation of the biological performance of the materials. Structural analysis showed that the addition of ZnO did not improve the crystallinity or the chain orientation of PLLA to the extent we expected. Although these are key parameters for the piezoelectric response, the piezoelectricity of the films increased significantly, mainly due to the contribution of ZnO nanoparticles, which enhanced the overall response through their inherent piezoelectricity and influence on shear deformation. Surface properties were successfully optimized. The addition of ZnO and plasma treatment increased hydrophilicity and surface roughness and enabled greater exposure of the nanoparticles. This improved the adhesion of human cells. At the same time, we achieved cell proliferation after piezostimulation with ultrasound. In piezostimulation testing, PLLA/ZnO films showed an antimicrobial effect, which was most pronounced at higher concentrations of ZnO, but these concentrations were also cytotoxic. The optimal balance between biocompatibility and antimicrobial activity was achieved at a concentration of 1 wt% ZnO, where the results for human cell growth and bacterial inhibition were the most favorable. organski piezoelektrik poli-L-laktid (PLLA) cinkov-oksid (ZnO) piezo-stimulacija organic piezoelectric poly-L-lactid (PLLA) zinc oxide (ZnO) piezo-stimulation true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-10-03 11:25:01 2025-10-03 11:25:12 2025-11-27 12:18:24 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 24213 258975747 Stor_Spela_-_Piezoelektricni_biomateriali_na_osnovi_poli-l-laktida,_modificirani_z_delci_cink-ok.pdf Stor_Spela_-_Piezoelektricni_biomateriali_na_osnovi_poli-l-laktida,_modificirani_z_delci_cink-ok.pdf 1 6E5D8D23D47FE18CEF4D0D27D4C6D16D c364e588b6467679ed9ca9871c8b79a3fafb58958f3af9e12b5740a7095634a5 a7e26ffd-a03a-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=219340 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0