20.500.12556/RUL-174289 Spremljanje interakcij med antagonističnimi mikroorganizmi iz biotičnih pripravkov Monitoring of the interactions of biological control products based on antagonistic microorganisms Naraščajoča svetovna populacija postavlja pred kmetijstvo velik izziv za zagotavljanje zadostne količine hrane, hkrati pa se krepi potreba po trajnostnih in okolju prijaznih pristopih pridelave hrane. Ena od obetavnih metod je biotično varstvo rastlin, ki temelji na uporabi antagonistov za zatiranje škodljivih mikroorganizmov. V magistrski nalogi smo preučevali interakcije med štirimi koristnimi organizmi: glive Trichoderma asperellum T34, Pythium oligandrum M1 in Clonostachys rosea (Gliocladium catenulatum J1446) in bakterija Bacillus amyloliquefaciens QST 713. Analizirali smo njihovo rast v dvojnih kulturah pri različnih temperaturah (15 °C in 25 °C) ter ob dveh terminih inokulacije (sočasna in z zamikom treh dni). Rezultati so pokazali, da so bile interakcije izrazito odvisne od temperature in časa inokulacije. Pri sočasni inokulaciji so posamezne vrste izražale različno intenziteto rasti glede na kombinacijo partnerja, pri čemer so bile razlike med 15 °C in 25 °C zelo izrazite. Pri inokulaciji z zamikom so imele prednost kulture, ki so bile na gojišče vnesene prve, vendar se je vpliv razlikoval glede na vrsto organizma. Najbolj konsistenten rezultat vseh obravnavanj je bila boljša rast pri višji temperaturi (25 °C). Ugotavljamo, da medsebojne interakcije med antagonisti niso univerzalne, temveč jih pomembno oblikujejo okoljski dejavniki in dinamika inokulacije. Rezultati naše raziskave ponujajo pomembne smernice za optimizacijo uporabe biotičnega varstva in prispevajo k razvoju trajnostnih praks v kmetijstvu. The rapidly growing global population poses a major challenge for agriculture to ensure sufficient food production while simultaneously emphasizing the need for sustainable and environmentally friendly approaches in food production. One promising method is biological control of plant pathogens, which relies on the use of antagonistic microorganisms. In this master’s thesis, we investigated the interactions among four beneficial organisms: fungi Trichoderma asperellum T34, Pythium oligandrum M1 and Clonostachys rosea (Gliocladium catenulatum J1446) and bacterium Bacillus amyloliquefaciens QST 713Their growth was analyzed in dual cultures at two temperatures (15°C and 25°C) and with two inoculation timings (simultaneous inoculation and a three-day delay). The results revealed that both temperature and inoculation timing had a significant influence on microbial interactions. Under simultaneous inoculation, growth intensity varied depending on the partner organism, with notable differences between 15°C and 25°C. In delayed inoculation experiments, the microorganisms inoculated first had a competitive advantage, although the extent of this advantage varied among species. Across all treatments, the most consistent observation was enhanced growth at the higher temperature (25°C). We conclude that interactions among antagonistic microorganisms are not universal but are strongly shaped by environmental conditions and inoculation dynamics. Understanding these factors is crucial for the effective implementation of biological control in agricultural practice. The findings provide valuable insights into the competitive behavior of antagonists and contribute to the development of sustainable plant protection strategies. biotično varstvo antagonisti Trichoderma asperellum Bacillus amyloliquefaciens Pythium oligandrum Clonostachys rosea temperatura inokulacija biological control antagonists Trichoderma asperellum Bacillus amyloliquefaciens Pythium oligandrum Clonostachys rosea temperature inoculation true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-10-01 07:15:08 2025-10-01 07:15:23 2025-11-17 04:07:08 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 267087 252081667 53958.pdf 53958.pdf 1 34113B677B613E2AA8A453956E7EF691 125d456e223719e07a467f2b4c912f3e6287d1265cce19b6a7256b6d93af377b 7c83a53d-9e85-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=219078 Biotehniška fakulteta 0 0 0