20.500.12556/RUL-173748 Bioinformatics analysis of miRNA-target gene interactions in chicken Bioinformacijska analiza interakcij miRNA in tarčnih genov pri kokoši MicroRNAs (miRNAs) are key post-transcriptional regulators of gene expression and are involved in numerous physiological and pathological processes. However, no com-prehensive map of miRNA-target interactions (MTIs) has yet been constructed for chicken, an important animal model in research. This study aimed to construct an inter-action network of the complete miRNA regulome in chicken by compiling all currently experimentally validated MTIs from miRTarBase and relevant literature, including phe-notype associations and differential expression data. We extracted 115 unique MTIs comprising 64 miRNAs and 81 target genes, and constructed an interaction network using Cytoscape. The network revealed that most MTIs are associated with development and various diseases, most of which related to cancer. Several miRNAs, such as gga-miR-23b-5p and gga-miR-199-5p, exhibited persistent upregulation across multiple car-diogenesis-related MTIs, indicating potential regulatory hubs. Target genes such as CTNNB1 were regulated by multiple miRNAs and were upregulated in ovarian cancer phenotypes. This work represents the first complete depiction of the chicken miRNA regulome and provides a framework for identifying key regulatory molecules in devel-opment and disease. Mikro RNA (miRNA) so ključni posttranskripcijski regulatorji izražanja genov, ki sodelujejo v številnih fizioloških in patoloških procesih. Kljub temu za kokoš, pomem-ben živalski model v raziskavah, še ni bila izdelana celovita karta interakcij med miRNA in tarčnimi geni (angl. miRNA-target gene interaction; MTI). Namen te študije je bil oblikovati interakcijsko mrežo celotnega miRNA reguloma pri kokoši z združitvijo vseh trenutno eksperimentalno potrjenih MTI iz podatkovne zbirke miRTarBase in ustrezne literature, vključno s podatki o fenotipih in diferencialni izraženosti. Pridobili smo 115 edinstvenih MTI, ki vključujejo 64 miRNA in 81 tarčnih genov, ter s programsko opremo Cytoscape izdelali mrežo interakcij. Mreža je pokazala, da je večina MTI-jev povezana z razvojem in boleznimi, predvsem z rakom. Nekatere miRNA, kot sta gga-miR-23b-5p in gga-miR-199-5p, sta imeli povišano izražanje v več MTI, povezanih s kardiogenezo, kar kaže na potencialna regulacijska stičišča. Tarčni geni, kot je CTNNB1, so uravnavani z več miRNA in so imeli povišano izražanje pri raku jajčnikov. To delo predstavlja prvo celovito predstavitev miRNA reguloma pri kokoši in pred-stavlja osnovo za identifikacijo ključnih regulatornih molekul pri razvoju in boleznih. miRNA target genes MTI network differential expression interaction hubs miRNA tarčni geni MTI mreža diferencialno izražanje interakcijska vozlišča true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-21 07:17:30 2025-09-21 07:17:34 2025-10-11 04:07:02 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 265258 249831683 53305.pdf 53305.pdf 1 AA8C80CB55FA0F3D6615AD1572A99FE3 d95156351fce4f14186da3a48fc067e9831105349fd9f48104fbe689220339f8 19aa077a-96aa-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=218400 Biotehniška fakulteta 0 0 0