20.500.12556/RUL-173587 Kemotaksični odziv parazitskih ogorčic (Rhabditidae) na izbrane sekundarne metabolite krompirja (Solanum tuberosum) Chemotactic response of parasitic nematodes (Rhabditidae) to selected secondary metabolites of potato (Solanum tuberosum) Parazitske ogorčice polžev (POP) predstavljajo učinkovito sredstvo za zatiranje škodljivih polžev v kmetijstvu. Uporaba koristnih organizmov, kot so POP, sodi v okvir biotičnega varstva rastlin, ki velja za okolju prijaznejšo alternativo kemičnim sredstvom. Ogorčice pri iskanju gostiteljev zaznavajo hlapne organske spojine (HOS), ki jih izločajo rastline, vendar so njihovi odzivi na posamezne HOS še slabo raziskani. Predpostavili smo, da je kemotaksični odziv POP na HOS pogojen z več dejavniki, kot so vrsta ogorčic, vrsta in koncentracija HOS, temperatura ter interakcije med navedenimi dejavniki. V raziskavi smo preučevali tri vrste POP: Phasmarhabditis papillosa, Oscheius myriophilus in Oscheius onirici. Namen raziskave je bil ugotoviti kemotaksični odziv teh vrst na HOS, ki jih izločajo poškodovani oziroma nepoškodovani gomolji krompirja (Solanum tuberosum L.). V ta namen smo vključili šest spojin, značilnih za poškodovane gomolje: [1] oktanal, [2] dekanal, [3] undekan, [4] nonanal, [5] 6-metil-5-hepten-2-on in [6] 1,2,4-trimetilbenzen, ter eno spojino, značilno za nepoškodovane gomolje: [7] 2-etil-1-heksanol. Rezultati so pokazali, da je bil oktanal najučinkovitejši atraktant, zlasti za vrsto O. myriophilus, medtem ko so ostale spojine izkazovale različen učinek. Privlačnost ogorčic je bila izrazitejša pri nižji temperaturi (18 °C) in višji koncentraciji HOS, kar kaže, da je kemotaksični odziv POP odvisen tudi od okoljskih dejavnikov. Izsledki poudarjajo potencial oktanala in 6-metil-5-hepten-2-ona za izboljšanje zatiranja polžev z uporabo ogorčic. Vendar pa lahko fizikalno-kemijske lastnosti tal in mikrobna aktivnost vplivajo na učinkovitost HOS, zato so potrebne nadaljnje raziskave za optimizacijo njihove uporabe na terenu in vključitev teh strategij v trajnostno varstvo rastlin. Slug-parasitic nematodes (SPN) represent an effective means of controlling harmful slugs in agriculture. The use of beneficial organisms, such as SPN, is part of biological plant protection, which is considered an environmentally friendly alternative to chemical pesticides. These nematodes use volatile organic compounds (VOCs) released by plants to locate their hosts; however, their responses to individual VOCs are still poorly understood. We hypothesized that the chemotactic response of SPN to VOCs is influenced by various factors, including nematode species, type and concentration of VOCs, temperature, and interactions among these variables. In our study, we investigated three SPN species: Phasmarhabditis papillosa, Oscheius myriophilus, and Oscheius onirici. The aim of the research was to examine the chemotactic responses of these species to VOCs emitted by damaged and undamaged potato tubers (Solanum tuberosum L.). To this end, we included six VOCs characteristic of damaged tubers: [1] octanal, [2] decanal, [3] undecane, [4] nonanal, [5] 6-methyl-5-hepten-2-one, and [6] 1,2,4-trimethylbenzene, as well as one VOC characteristic of undamaged tubers: [7] 2-ethyl-1-hexanol. The results showed that octanal was the most effective attractant, particularly for O. myriophilus, while the other compounds exhibited variable effects. Nematode attraction was more pronounced at lower temperatures (18 °C) and higher VOC concentrations (pure compound), suggesting that the chemotactic response of SPN is also influenced by environmental conditions. These findings highlight the potential of octanal and 6-methyl-5-hepten-2-one to enhance slug control through the use of nematodes. However, soil physicochemical properties and microbial activity may affect VOC efficacy, indicating the need for further research to optimize field application and to integrate these strategies into sustainable plant protection. kemotaksični odziv parazitska ogorčica Oscheius myriophilus Phasmarhabditis papillosa Oscheius onirici sekundarni metaboliti koncentracija temperatura usmerjeno gibanje krompir chemotactic respone parasitic nematode Oscheius myriophilus Phasmarhabditis papillosa Oscheius onirici secondary metabolits concentration temperature directed movement potato true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-09-19 07:15:09 2025-09-19 07:15:25 2025-11-11 03:37:57 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 264925 249669891 50565.pdf 50565.pdf 1 51A67317ADB987E05197F1ACF8CC0C2C 70fab6d4af0ac376a07079d8055dd7fb17f0d79b8277a39d3599f6cb762be0ee 8a290492-9517-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=218191 Biotehniška fakulteta 0 0 0