20.500.12556/RUL-173401 Vrednotenje vpliva kozmetičnih izdelkov z ekstraktom mikroalge Dunaliella species na izbrane parametre kože Evaluation of the effect of cosmetic products with incorporated microalgae Dunaliella species extract on selected skin parameters Koža, človekov največji organ, ima številne pomembne vloge, od katerih je zlasti zaščitna funkcija kože ena najpomembnejših, kar je v neposredni povezavi s tem, da predstavlja bariero med notranjostjo in zunanjostjo telesa. Telo ščiti pred raznovrstnimi zunanjimi dejavniki ter prekomernim izhlapevanjem vode ter vdorom snovi v kožo. Pri tem ima ključno vlogo zlasti rožena plast povrhnjice kot najbolj zunanja plast kože, hkrati pa pomembno vpliva na zunanji videz kože in je tako relevantna tudi s kozmetičnega vidika. Blagodejni vplivi ekstrakta mikroalge Dunaliella species na lastnosti kože so že bili potrjeni v predhodnih raziskavah. V okviru diplomske naloge smo slednjega vgradili v kozmetične formulacije in spremljali vpliv hidrofilne kreme in tekočih kristalov brez in z vgrajenim ekstraktom alg na izbrane parametre kože 15 prostovoljk tekom 14 dnevne uporabe. Dodatno smo spremljali tudi vpliv predhodnega toniziranja kože s slanico, ki mu je sledil nanos formulacij z vgrajenim ekstraktom alg. Stanje kože smo ovrednotili z uporabo neinvazivnih instrumentalnih metod, in sicer smo spremljali spremembe v vrednostih transepidermalne izgube vode, melaninskega in eritemskega indeksa, hidratacije in pH kože ter biomehanskih parametrov kože. V naši študiji smo opazili, da je transepidermalna izguba vode (TEWL) narasla po uporabi vzorcev na osnovi tekočih kristalov oz. klasičnih emulzijskih gelov testiranih izdelkov, kot tudi pri sočasni uporabi slanice. To je v nasprotju s predhodnimi rezultati in tudi rezultati merjenja eritemskega indeksa. Najnižje vrednosti slednjega smo namreč izmerili po negi kože s slanico in hidrofilno kremo z algnim ekstraktom. Po dvotedenski uporabi hidrofilne kreme brez ali z algnim ekstraktom smo opazili povečanje hidratiranosti kože prostovoljk, tudi v primeru predhodne uporabe slanice. Pri uporabi izdelkov na osnovi tekočih kristalov to nismo zaznali. So pa imeli slednji ugodnejši učinek na pH kože, ki se po nanosu izdelkov na osnovi tekočih kristalov ni spremenil oz. se je celo nekoliko znižal. Pri nekaterih vzorcih smo zaznali tudi pozitiven vpliv na izbrane biomehanske parametre kože. Rezultati diplomske naloge potrjujejo smotrnost sočasne uporabe vlažilne kreme z vgrajenim ekstraktom mikroalge Dunaliella species kot kozmetično aktivne sestavine in slanice, predvsem pa pomembno doprinašajo k nadaljnjemu razvoju kozmetičnih formulacij za nego kože z vidika mikrostrukture. The skin, the largest organ of the human body, plays numerous important roles, with its protective function being one of the most crucial. This is directly related to the fact that the skin acts as a barrier between the internal and external environments of the body. It protects the body from various external factors, excessive water evaporation, and the penetration of substances into the skin. In this context, the stratum corneum, the outermost layer of the epidermis, plays a key role. It also significantly influences the skin’s appearance, making it relevant from a cosmetic perspective. The beneficial effects of the microalgae Dunaliella species extract on skin properties have already been confirmed in previous studies. In this thesis, we incorporated the extract into cosmetic formulations and observed the effects of a hydrophilic cream and liquid crystals, both with and without the incorporated algae extract, on selected skin parameters in 15 female volunteers over a 14-day period. Additionally, we monitored the effects of pre-toning the skin with saline solution, followed by the application of formulations containing the algae extract. The skin condition was evaluated using non-invasive instrumental methods. Specifically, we measured changes in transepidermal water loss (TEWL), melanin and erythema index, skin hydration, pH, and biomechanical skin parameters. In our study, we observed an increase in TEWL after using the liquid crystal-based or classical emulsion gel products, as well as with the concurrent use of saline. This contradicts previous findings and also the results of the erythema index measurements. The lowest erythema values were recorded after skin treatment with saline and the hydrophilic cream containing the algae extract. After two weeks of using the hydrophilic cream, both with and without the algae extract, an increase in skin hydration was observed in the volunteers, even when saline was used beforehand. This effect was not observed with the liquid crystal-based products. However, these products had a more favorable effect on skin pH, which did not change or slightly decreased after application. In some samples, we also detected a positive influence on selected biomechanical parameters of the skin. The results of this thesis confirm the beneficial effects of using a moisturizing cream containing Dunaliella species microalgae extract as a cosmetically active ingredient in combination with saline. Most importantly, they contribute significantly to the further development of cosmetic formulations for skin care from a microstructural perspective. Rožena plast TEWL hidratacija melaninski in eritemski indeks pH Stratum corneum TEWL hydration melanin and erythema index pH true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-17 07:45:46 2025-09-17 07:45:49 2025-11-08 03:58:08 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 118169 28491.pdf 28491.pdf 1 E1E0E627143E377A6972D65A4C92C8E0 f26d8b94e2d7edd6c516bbf4be2f6c681133766a8bad862cea2a30b00efc74a1 63c7a927-9389-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217811 Fakulteta za farmacijo 0 0 0