20.500.12556/RUL-173339 Development of sustainable biocatalytic furfurylamine production in a magnetic field-assisted microfluidic reactor The increasing demand for furfurylamine (FA), a versatile biobased building block, necessitates the development of efficient and sustainable production processes. This study presents a continuous biocatalytic process for the amination of furfural (FUR) to FA, aligning with green chemistry principles and circular economy strategies. A systematic screening of ω-transaminases (ω-TAs) and amine donors identified N-His$_6$-ATA-wt and (S)-(−)-α-methylbenzylamine as the optimal pair, achieving a 96% FA gross yield within 30 min at equimolar substrate concentrations, surpassing previously reported ω-TA-based FA productions. To enable biocatalyst long-term use in continuous processes, the enzyme was covalently immobilized on synthesized and functionalized magnetite nanoparticles (MNPs) using glutaraldehyde (GA) as a cross-linker. At optimized immobilization conditions, 92.8% recovered activity was achieved with 80 mg enzyme/g dry MNPs and 2% (v/v) GA in a batch process. The immobilized biocatalyst was integrated into a custom 3D-printed magnetic field-assisted microreactor and evaluated in continuous-flow operation for 18 days. The system reached a maximum space-time yield of 1.07 g/(L h) and a total turnover number of 2.04 × 10$^7$. These results, along with favorable green chemistry metrics, highlight the potential of this integrated approach─combining enzyme engineering, nanomaterials, and flow technology─for scalable and sustainable FA production. biocatalysis microreactor furfurylamine biomass valorization furfural ω-transaminase magnetite nanoparticles enzyme immobilization green chemistry amines nanoparticles peptides proteins phosphates biokataliza mikroreaktor furfurilamin valorizacija biomase true false true Angleški jezik Slovenski jezik Članek v reviji 2025-09-16 09:44:59 2025-09-16 09:45:00 2025-12-06 04:45:12 0000-00-00 00:00:00 2025 0 0 Str. 15887-15896 iss. 38 Vol. 13 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.023.2.098:547.7 2168-0485 10.1021/acssuschemeng.5c04752 249049091 development-of-sustainable-biocatalytic-furfurylamine-production-in-a-magnetic-field-assisted-microfluidic-reactor.pdf development-of-sustainable-biocatalytic-furfurylamine-production-in-a-magnetic-field-assisted-microfluidic-reactor.pdf 1 13337A55F75F1C911E2E13AC6B096118 72c6931a2904b0740e9b55a5eb82c098d4caa78acec8fcdad12fad115e139222 9b8e7641-d1c5-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=223066 https://pubs.acs.org/doi/10.1021/acssuschemeng.5c04752 1 e0e0e6c3-92d0-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217691 Fakulteta za kemijo in kemijsko tehnologijo Sustainable furfural valorization Sustainable furfural valorization 0 0 0