20.500.12556/RUL-173207 Razširjenost glive Dothistroma pini v Sloveniji in vpogled v prisotnost njenih paritvenih tipov Distribution of the fungus Dothistroma pini and its mating types in Slovenia Glavni cilj magistrskega dela je bil preučiti pojavnost glive Dothistroma pini Hulbary in njenih paritvenih tipov v Sloveniji. Gliva D. pini skupaj s sorodno glivo Dothistroma septosporum (Dorogin) M. Morelet povzroča bolezen rdečo pegavost borovih iglic. Intenziteta in razširjenost omenjene bolezni borovih iglic se zadnji dve desetletji povečujeta, bolezen je v Sloveniji prisotna po celotni državi, zlasti pa v sestojih črnega bora na Krasu. V prvem delu raziskave smo analizirali molekularne podatke 165 arhiviranih vzorcev, ki jih je Oddelek za varstvo gozdov Gozdarskega inštituta Slovenije zbral med letoma 2011 in 2022 in v katerih je bila potrjena prisotnost vsaj ene povzročiteljice bolezni. Rezultat analize preteklih vzorčenj je baza podatkov potrjenih lokacij gliv D. pini ter D. septosporum ter paritvenih tipov glive D. pini. Ugotovili smo, da je gliva D. pini splošno prisotna v Sloveniji, vendar je njena populacija manjša in prostorsko bolj omejena v primerjavi z D. septosporum. Paritveni tip MAT1-2 glive D. pini je prevladujoč, vendar je bil pogosteje kot so sprva nakazovale raziskave zaznan tudi tip MAT1-1. V drugem delu raziskave smo opravili popis osutosti ter vzorčenje 15 simptomatskih borov na treh lokacijah (Pivka, Divača, Pliskovica), kjer je bila gliva D. pini v preteklosti že potrjena. Z molekularnimi analizami smo ponovno potrdili prisotnost D. pini na vseh treh lokacijah, v večini vzorcev je bil zaznan paritveni tip MAT1-2, v enem vzorcu iz Pivke pa smo določili tudi tip MAT1-1. V tretjem delu raziskave smo neposredno iz trosišč iz simptomatskih iglic uspeli izolirati šest čistih kultur glive D. pini, vsem je bil določen paritveni tip MAT1-2. The main goal of the thesis was to analyse the occurrence of the fungus Dothistroma pini Hulbary and its mating types in Slovenia. Together with Dothistroma septosporum (Dorogin) M. Morelet, it causes Dothistroma needle blight, a disease whose intensity and distribution have been increasing over the past two decades and is now widespread across the country, especially in black pine stands of the Karst region. In the first part of the study, we analysed molecular data from 165 archived samples collected between 2011 and 2022 by the Department of Forest Protection of the Slovenian Forest Institute, in which at least one of the target pathogens was detected. The result is a database of confirmed locations of D. pini and D. septosporum and the occurrence of D. pini mating types. We found that D. pini is generally present in Slovenia, although its population is smaller and more spatially restricted compared to D. septosporum. The MAT1-2 mating type of D. pini is predominant; however, the MAT1-1 type was also detected more frequently than previous studies indicated. In the second part of the study, we conducted needle loss assessments and sampled 15 symptomatic pine trees at three selected sites (Pivka, Divača, Pliskovica) where D. pini had previously been confirmed. Molecular analyses reconfirmed the presence of D. pini at all three sites, predominantly with MAT1-2 and a single detection of MAT1-1 mating type in a sample from Pivka. In the third part of the study, we isolated six pure cultures of D. pini directly from sporulating symptomatic needles. All cultures were molecularly confirmed as D. pini with the MAT1-2 mating type. bori rdeča pegavost borovih iglic Dothistroma pini Dothistroma septosporum paritveni tip bolezni borov pines Dothistroma needle blight Dothistroma pini Dothistroma septosporum mating type pine diseases true false false [M. Kadunc] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-09-14 07:15:08 2025-09-14 07:15:42 2025-11-06 03:44:38 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*17:630*44(043.2)=163.6 264304 249097731 51017.pdf 51017.pdf 1 D33F9F7961467A15E79B19A3A741DFF8 d02df99334d93cc99d3b6d74275b5409e512e3fb875639ff39e4656233dd900c b25b08e6-9129-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217379 51018.pdf 51018.pdf 1 0F0147F6B78E2B20759C5DE24DABFC75 4c110b7d70e06827d9cce1516b8c6c77e634e775d048b66c82d598dbf4a2849d b25b9d17-9129-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217380 Biotehniška fakulteta 0 0 0