20.500.12556/RUL-172947 Vrednotenje izvlečka alg Dunalliela species in betametazondipropionata s protivnetnim delovanjem za dermalno uporabo Evaluation of Dunalliela species algae extract and betamethasone dipropionate with anti-inflammatory activity for dermal application Koža je stalno izpostavljena škodljivim okoljskim dejavnikom, predvsem onesnaženemu zraku, ki oslabi njeno barierno funkcijo in poveča oksidativni stres. Mikroalga Dunaliella species, bogata z antioksidanti, se preučuje kot obetavna sestavina v dermokozmetičnih izdelkih za zaščito kože pred oksidativnimi poškodbami ter krepitvijo kožne bariere. V diplomski nalogi smo v prvem sklopu dela preučili vpliv izvlečka Dunaliella species na človeško celično linijo keratinocitov (NCTC2544). V primerjalne namene smo vključili tudi druge mikroorganizme, kot sta mikroalga Tetradesmus species in cianobakterija Phormidium species. Nadalje smo v drugem sklopu dela preučili še napredne dostavne sisteme, kot so liposomi in transferosomi, brez in z vgrajenim betametazondipropionatom, lokalnim kortikosteroidom, namenjenem zdravljenju vnetnih kožnih bolezni. Najprej smo izvedli fizikalno-kemijsko vrednotenje teh sistemov, ki je vključevalo določanje velikosti delcev, polidisperznega indeksa in zeta potenciala, s čimer smo ocenili stabilnost in tehnološke lastnosti izdelanih formulacij. Po tem smo preučili tudi njihov vpliv na izbrano celično linijo. V obeh sklopih dela smo izvedli test za določanje celične metabolne aktivnosti z reagentom MTS, ki odraža metabolno aktivnost živih celic in je posredno povezan s celično proliferacijo, ter preučili celično morfologijo z invertnim svetlobnim mikroskopom. V okviru vrednotenja vzorcev mikroalg in cianobakterij, je bila pri vseh testiranih koncentracijah (1, 5, 10 in 15 µL/mL) dosežena celična proliferacija 100 % ali več, brez pomembnih razlik med posameznimi sevi. V primerjavi s standardnim iritantom (natrijev dodecil sulfat), z opazno nižjo (≤5,1 %) proliferacijo, so bili vsi testirani vzorci bistveno bolj biokompatibilni. Analiza celične morfologije je potrdila te ugotovitve, saj so celice ohranjale značilno rast in morfologijo brez znakov poškodb. Izdelani liposomi in transferosomi so izkazovali primerne fizikalno-kemijske lastnosti, izkazali pa so se tudi z vidika biokompatibilnosti pri vseh testiranih volumnih disperzije (1–4 µL/vdolbinico), tako brez kot z vgrajenim betametazondipropionatom. Vgradnja kortikosteroida je sicer nekoliko znižala celično proliferacijo, kar pripisujemo njegovemu antiproliferativnemu delovanju. Liposomi (≥94,0 %) so dosegli nekoliko boljše rezultate kot transferosomi (≥88,3 %), vendar sta obe formulaciji izkazali bistveno višjo celično proliferacijo v primerjavi s standardnim iritantom (≤3,8 %). Mikroskopska analiza celične morfologije je potrdila tudi ohranjeno rast in značilno obliko celic brez znakov poškodb. The skin is constantly exposed to harmful environmental factors, especially air pollution, which weakens its barrier function and increases oxidative stress. The antioxidant-rich microalgae Dunaliella species is being studied as a promising ingredient in dermocosmetic products designed to protect the skin from oxidative damage and strengthen the skin barrier. In the first part of our thesis, we studied its effects on a human keratinocyte cell line (NCTC2544), alongside microalgae Tetradesmus species and cyanobacterium Phormidium species for comparison. Furthermore, in the second part of the study, we examined advanced delivery systems such as liposomes and transferosomes, both with and without betamethasone dipropionate incorporated, a local corticosteroid commonly used in the treatment of inflammatory skin diseases. First, we performed a physical-chemical evaluation of these systems, which included determining particle size, polydispersity index, and zeta potential, thereby assessing the stability and technological properties of the formulations produced. We then examined their effect on the selected cell line. In both parts of the work, we performed a test to determine cell metabolic activity using the MTS reagent, which reflects the metabolic activity of living cells and is indirectly related to cell proliferation, and examined cell morphology using an inverted light microscope. In the evaluation of microalgae and cyanobacteria samples, cell proliferation of 100 % or more was achieved at all tested concentrations (1, 5, 10, and 15 µL/mL), with no significant differences between individual strains. Compared to the standard irritant (sodium dodecyl sulfate), where proliferation was noticeably lower (≤5.1 %), all tested samples were significantly more biocompatible. Cell morphology analysis confirmed these findings, as the cells maintained their characteristic growth and morphology without signs of damage. The liposomes and transferosomes produced exhibited suitable physicochemical properties and also proved to be biocompatible at all tested dispersion volumes (1–4 µL/well), both with and without betamethasone dipropionate incorporated. The incorporation of the corticosteroid slightly reduced cell proliferation, which we attribute to its antiproliferative effect. Liposomes (≥94.0 %) achieved slightly better results than transferosomes (≥88.3 %), but both formulations showed significantly higher cell proliferation compared to the standard irritant (≤3.8 %). Microscopic analysis of cell morphology also confirmed preserved growth and characteristic cell shape without signs of damage. citotoksičnost morfologija celic liposomi transferosomi cytotoxicity cell morphology liposomes transferosomes true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-12 07:45:12 2025-09-12 07:45:14 2025-10-29 03:34:54 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 117593 28751.pdf 28751.pdf 1 123794A2A01284AF461E37F83DC9DC88 6906d7fb41a4d1909d7dd96657b073a5ed6556e38f14c97aa7187d59a3d797b3 7db21d79-8f9b-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217082 Fakulteta za farmacijo 0 0 0