20.500.12556/RUL-172933 Analiza lokalnih genskih virov tatarske ajde z mikrosatelitnimi označevalci Analisys of local genetic sources of Tartary buckwheat with microsatellite markers V diplomski nalogi smo se osredotočili na genotipizacijo tatarske ajde (Fagopyrum tataricum Gaertn.), pri čemer smo analizirali 14 različnih genskih virov. Od teh je bilo 7 lokalnih in 7 tujih, ki se jih hrani v genski banki Biotehniške fakultete Univerze v Ljubljani. S postopkom izolacije smo iz listov pridobili kakovostno DNA, ki smo jo z 8 mikrosatelitnimi označevalci vključili v verižno reakcijo s polimerazo. Za preverjanje in vizualizacijo namnoženih fragmentov DNA smo uporabili agarozno gelsko elektroforezo, ki omogoča analizo prisotnosti in velikosti fragmentov, uspešno se je pomnožilo vseh 8 lokusov. Nato smo s kapilarno elektroforezo pridobili podatke, ki smo jih obdelali s programom GeneMapper 6. Parametre genetske variabilnosti smo izračunali s programom Cervus. Uspešno se je pomnožilo 20 različnih alelov, v povprečju 2,5 alela na lokus. Povprečna informacijska vrednost polimorfizma (PIC) je bila 0,341. Najkrajši alel je imel dolžino 141 bp, najdaljši pa 267 bp. Povprečna dejanska heterozigotnost (Ho) je bila 0,5, povprečna pričakovana heterozigotnost (He) pa 0,4. Trije SSR označevalci od 8 so bili monomorfni, kar nakazuje na visoko homozigotnost pri proučevanih genotipih. Zelo dobro razmejeno skupino, ki jo potrjuje visoka statistična podpora (bootstrap), so tvorili 4 genotipi: 122, B, 105 in 132. In the thesis, we focused on the genotyping of Tartary buckwheat (Fagopyrum tataricum Gaertn.), analyzing 14 different genetic resources. Of these, 7 were local and 7 were foreign, all stored in the gene bank of the Biotechnical Faculty, University of Ljubljana. Using the isolation procedure, we extracted high-quality DNA from leaves, which was then amplified with 8 microsatellite markers using polymerase chain reaction (PCR). To verify and visualize the amplified DNA fragments, we used agarose gel electrophoresis, which enables the analysis of fragment presence and size. All 8 loci were successfully amplified. Subsequently, capillary electrophoresis was used to obtain data, which were processed with the GeneMapper 6 software. Genetic variability parameters were calculated using Cervus software. A total of 20 different alleles were successfully amplified, with an average of 2.5 alleles per locus. The average polymorphism information content (PIC) was 0.341. The shortest allele measured at 141 bp, while the longest was 267 bp. The average observed heterozygosity (Ho) was 0.5, slightly higher than the average expected heterozygosity (He) of 0.4. Three out of the 8 SSR markers were monomorphic, indicating a high level of homozygosity in the studied genotypes. A well-defined group, strongly supported by a high bootstrap value, was formed by 4 genotypes: 122, B, 105, and 132. tatarska ajda lokalni genski viri DNA lokus alel mikrosatelitni označevalci genotipizacija dendrogram faktorialna analiza Tartary buckwheat local genetic sources DNA locus allele microsatellite markers genotypization dendrogram factorial analysis true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-12 07:19:24 2025-09-12 07:19:26 2025-10-28 03:54:34 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 263672 249018371 51694.pdf 51694.pdf 1 DF2EB7CB108C3F2ED180D9C5F76A87C6 4da2b7220088767316e55879272df14457ab140eb4babc3ab95f08456d82bb36 e31196ea-8f97-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217067 Biotehniška fakulteta 0 0 0