20.500.12556/RUL-172919 Odkrivanje novih bioaktivnih spojin pri rodu Streptomyces z aktivacijo kriptičnih biosintetskih genskih gruč Discovery of novel bioactive compounds in Streptomyces through activation of cryptic biosynthetic gene clusters Namen diplomskega dela je predstaviti rod Streptomyces, ki predstavlja izjemno pomemben vir bioaktivnih spojin, saj večina vseh komercialno dostopnih antibiotikov proizvedejo predstavnikov tega rodu. Kljub obsežnemu biosintetskemu potencialu ostaja približno 90% biosintetskih genskih gruč (BGC), ki kodirajo za tovrstne učinkovine, kriptičnih v standardnih laboratorijskih in fermentacijskih pogojih. Tukaj so predstavljene različne strategije za odkrivanje in aktivacijo kriptičnih biosintetskih genskih gruč za sintezo novih bioaktivnih spojin. Bioinformatični pristopi omogočajo napoved strukture in funkcije BGC ter določanje prioritet za eksperimentalno validacijo. Metabolni inženiring zamenjuje regulatorne elemente ter premaguje regulatorne genetske omejitve, kar poveča izkoristek in omogoča sintezo novih, prej neznanih naravnih produktov. In-situ metode omogočajo aktivacijo poti v naravnem gostitelju, pri čemer so bili doseženi primeri tvorbe novih antibiotikov. Lahko sklepamo, da predstavljena kombinacija bioinformatike in eksperimentalnih pristopov bistveno poveča možnosti odkrivanja novih naravnih produktov s potencialno pomembnimi farmakološkimi lastnostmi, kar je ključno pri spopadanju z naraščajočo odpornostjo proti antibiotikom. The purpose of the thesis is to present the genus Streptomyces, which represents an extremely important source of bioactive compounds, as the majority of all commercially available antibiotics are produced from this genus. Despite the extensive biosynthetic potential, approximately 90% of biosynthetic gene clusters (BGCs) encoding for natural products remain cryptic under standard conditions. Different strategies for the discovery and activation of cryptic biosynthetic gene clusters are presented for the synthesis of novel bioactive compounds. Bioinformatic approaches allow for the prediction of the structure and function of BGCs and the priority with which they are to be experimentally validated. Metabolic engineering replaces regulatory elements and overcomes regulatory hurdles, which increases yield and enables the synthesis of new, previously unknown natural products. In-situ methods allow for the activation of the cryptic biosynthetic pathways in the natural host, whereby examples of the formation of new antibiotics have been achieved. We can conclude that the presented combination of bioinformatics and experimental approaches significantly increases the possibilities of discovering new natural products with potentially important pharmacological properties, which is crucial in combating the growing resistance to antibiotics. Streptomyces bioaktivne spojine BGC sekundarni metaboliti metabolni inženiring Streptomyces bioactive compounds BGC secondary metabolites metabolic engineering true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-12 07:18:44 2025-09-12 07:18:46 2025-10-28 03:45:52 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 263658 249165571 51142.pdf 51142.pdf 1 40C51F7C86730B29AFF937F95168FE17 b27a155b9c9cded364178618856a72dda1297aa4f80748e1a3468ac0dadec2a1 cb4fea24-8f97-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217053 Biotehniška fakulteta 0 0 0