20.500.12556/RUL-172882 Host trees and geographic distribution of the phylogenetic groups within the Fomes fomentarius species complex Drevesni gostitelji in geografska razširjenost filogenetskih skupin kompleksa vrst Fomes fomentarius Molecular barcoding has revealed distinct evolutionary lineages and the presence of cryptic species within the Fomes fomentarius species complex. As previous studies were limited in geographical and ecological coverage, this study aimed to fill these gaps by integrating broad spatial and host sampling with extensive molecular data analysis. To achieve this, we collected 200 new sporocarps from 15 countries and combined them with sequences from GenBank, resulting in a dataset of 749 ITS rDNA sequences. We confirmed 4 previously known phylogenetic lineages (A1, A2, B1, B2) within F. fomentarius s.l., and no new lineages were found in Europe, suggesting that the current diversity is well represented by the ITS data. Additionally, we detected possible new lineages in Asia and Africa, highlight the importance of further research in those regions. The distribution of phylogenetic lineage B (phylogenetic network haplotype group G1) is in the Mediterranean and lowlands of Central Europe, while phylogenetic lineage A (phylogenetic network haplotype group G5) was more common at higher elevations and in boreal climates. Isolates from Poland and Germany did not show altitude-based patterns but differed in host associations. Group G1 was distributed as an ecological generalist, while group G5 was mostly associated with Betula and Fagus. These findings highlight the importance of combining molecular and ecological data when studying fungal diversity. Molekularno črtno kodiranje je razkrilo različne evolucijske linije in prisotnost kriptičnih vrst znotraj kompleksa vrst Fomes fomentarius. Dosedanje študije so bile omejene z geografskega in ekološkega vidika, zato smo z raziskavo želeli zapolniti te vrzeli z vključitvijo širokega prostorskega vzorčenja na več gostiteljih in analizo razpoložljivih molekularnih podatkov. Analizirali smo 200 novih trosnjakov iz 15 držav in jih združili z javno dostopnimi nukleotidnimi zaporedji iz baze podatkov GenBank, kar je skupaj tvorilo nabor 749 ITS rDNA zaporedij. Potrdili smo štiri predhodno znane filogenetske linije (A1, A2, B1, B2) F. fomentarius s.l., pri čemer v Evropi nismo našli novih linij. To nakazuje, da je raznolikost, temelječa na markerju ITS, v Evropi dobro zajeta. Nasprotno smo za podatke iz Azije in Afrike ugotovili nove filogenetske linije, kar poudarja pomen nadaljnjih raziskav v teh regijah. Filogenetska linija B (skupina G1 v analizi filogenetskih mrež) je pogostejša v sredozemskem območju in nižinah Srednje Evrope, medtem ko je filogenetska linija A (skupina G5 v analizi filogenetskih mrež) prevladovala na višjih nadmorskih višinah in v borealnih klimatih. Izolati iz Poljske in Nemčije niso kazali višinskih vzorcev, temveč razlike v povezavi z gostiteljskimi drevesi. Skupina G1 se je obnašala kot ekološki generalist, medtem ko je bila skupina G5 večinoma odkrita na vrstah iz rodov Betula in Fagus. Ugotovitve potrjujejo pomen združevanja molekularnih in ekoloških podatkov pri preučevanju raznolikosti gliv. Fomes (tinder fungus) wood decomposing fungi molecular barcoding phylogenetic lineages phylogenetic network biodiversity host specificity Fomes (kresilke) lesne glive molekularno črtno kodiranje filogenetske linije filogenetska mreža biotska raznovrstnost gostiteljska specifičnost true false false [R. Logar] Angleški jezik Slovenski jezik Magistrsko delo/naloga 2025-09-12 07:16:41 2025-09-12 07:16:48 2025-10-27 03:31:56 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 630*17(043.2)=163.6 263636 248729347 50963.pdf 50963.pdf 1 0AB6B4C30DC9BD27DD8670B2D23A8DAC 60d2dd4322c5f09f1c4b84894aaa607f163977490b16317c6e3f545bc36c63ac 866453d8-8f97-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217016 Biotehniška fakulteta 0 0 0