20.500.12556/RUL-172672 Fotozamreževanje sintetičnih polipeptidov za pripravo gelov Photocrosslinking of synthetic polypeptides for hydrogel formation V magistrskem delu je predstavljen razvoj novega načina fotozamreževanja sintetičnih polipeptidov za pripravo gelov. Opisana je priprava polipeptidov s polimerizacijo N-karboksianhidridov α-aminokislin, njihova funkcionalizacija z izbranimi fotoodzivnimi skupinami ter izvedba različnih fotokemičnih zamreževalnih reakcij. Posebna pozornost je namenjena uvedbi alkena, tetrazola in maleimida, ki omogočajo izvedbo fotocikloadicij (npr. NITEC in tiol-en reakcija). V eksperimentalnem delu sem sintetizirala in funkcionalizirala polipeptide, jih okarakterizirala z NMR, MALDI-TOF in UV-Vis spektroskopijo ter proučevala zamreževanje z LED obsevanjem. Nastale gele sem analizirala z IR spektroskopijo in fotoreometrom ter raziskala njihovo stabilnost v globokih evtektičnih topilih kot okolju prijazni alternativi konvencionalnim topilom. This master's thesis presents the development of a novel approach for the photocrosslinking of synthetic polypeptides for hydrogel formation. It describes the synthesis of polypeptides via ring-opening polymerization of N-carboxyanhydrides of α-amino acids, their functionalization with selected photoresponsive groups, and the application of various photochemical crosslinking reactions. Particular attention is given to the incorporation of alkene, tetrazole, and maleimide moieties, which enable photocycloaddition reactions (e.g., NITEC and thiol-ene reactions). In the experimental part, polypeptides were synthesized, functionalized, and characterized using NMR spectroscopy, MALDI-TOF mass spectrometry, and UV-Vis spectroscopy. Crosslinking was investigated under LED irradiation. The resulting hydrogels were analyzed by IR spectroscopy and photorheometry, and their stability was studied in deep eutectic solvents as environmentally friendly alternatives to conventional organic solvents. N-karboksianhidridi α-aminokislin polimerizacija z odpiranjem obroča sintetični polipeptidi fotocikloadicija globoka evtektična topila α-amino acids N-carboxyanhydrides ring-opening polymerization synthetic polypeptides photocycloaddition deep eutectic solvents true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-09-10 14:10:01 2025-09-10 14:10:06 2025-11-13 14:31:49 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 24110 257122563 Planinc_Lara_-_Fotozamrezevanje_sinteticnih_polipeptidov_za_pripravo_hidrofobnih_gelov.pdf Planinc_Lara_-_Fotozamrezevanje_sinteticnih_polipeptidov_za_pripravo_hidrofobnih_gelov.pdf 1 AF94C7DF98F0106223348277514BBF04 9a88d2811e497c4712225afc7eea0be0805a5ef8d452ebb213da1b46f0058277 edc90656-8e3e-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=216672 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0