20.500.12556/RUL-172576 Vezava dietilenglikola na fluorescenčno molekulsko sondo Binding of diethylene glycol to a fluorescent molecular probe Amiloidi so vrsta proteinov, ki tvorijo agregate v obliki fibril. Njihova sekundarna struktura je bogata z β-ploskvami. Te predstavljajo tarčno vezavno mesto za fluorescenčne molekulske sonde, ki so lahko preko biotin–streptavidin interakcije vezane na magnetne nanodelce in se med drugim uporabljajo v raziskavah zgodnje detekcije nekaterih nevrodegenerativnih bolezni. V diplomski nalogi je predstavljena vezava izbrane fluorescenčne molekulske sonde na biotin preko dietilenglikolnega vmesnika. Sinteza je potekala v več stopnjah. Iz terc-butil (2-(2-(2-hidroksietoksi)etoksi)etil) karbamata in anhidrida jantarne kisline smo najprej sintetizirali vmesnik. Nanj smo s Steglichovim estrenjem vezali izbrano fluorescenčno molekulsko sondo, (E)-2-(metil(4-(2-nitrovinil)fenil)amino)etan-1-ol, ki smo jo s Henryjevo reakcijo pripravili iz 4-((2-hidroksietil)(metil)amino)benzaldehida. Dobili smo N-Boc zaščiteno spojino, na katero smo v zadnji stopnji po odstranitvi Boc zaščite vezali še biotin, ki smo ga aktivirali s pomočjo reagenta PyBOP. Amyloids are a type of protein that forms fibril-like aggregates. Their secondary structure is rich in β-sheet motifs. These represent a target binding site for fluorescent molecular probes, which can be attached to magnetic nanoparticles via biotin–streptavidin interaction and used in research on early detection of certain neurodegenerative diseases. This bachelor’s thesis presents the binding of a fluorescent molecular probe to biotin via a diethylene glycol linker, which was achieved through a multiple-step approach. First, a linker was synthesized using tert-butyl (2-(2-(2-hydroxyethoxy)ethoxy)ethyl)carbamate and succinic anhydride. A fluorescent molecular probe, (E)-2-(methyl(4-(2-nitrovinyl)phenyl)amino)ethan-1-ol, prepared from 4-((2-hydroxyethyl)(methyl)amino)benzaldehyde by the Henry reaction, was then coupled to it via Steglich esterification. This yielded an N-Boc protected compound to which biotin, activated by PyBOP reagent, was bound after the removal of a Boc protecting group. sinteza dietilenglikol fluorescenčna molekulska sonda biotin synthesis diethylene glycol fluorescent molecular probe biotin true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-09 09:55:00 2025-09-09 09:55:04 2025-10-30 10:26:59 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25549 255372035 Krasovec_Nina_-_Vezava_dietilenglikola_na_fluorescencno_molekulsko_sondo.pdf Krasovec_Nina_-_Vezava_dietilenglikola_na_fluorescencno_molekulsko_sondo.pdf 1 40A5CB23511BE618E7DA632EFCB483B6 b22aeb93f655575da14aba2ea184a63e98c314f1e5f9fd51f17b146e7c91e50e 2423402b-8d52-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=216457 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0