20.500.12556/RUL-172575 Podaljšanje fluorescenčne molekulske sonde z etilenglikolom Elongation of fluorescent molecular probe with ethylene glycol Fluorescenčne molekule imajo že od petdesetih let prejšnjega stoletja veliko vlogo v bioanalitiki. V zadnjih letih so bili razviti novi načini opremljanja fluorescenčnih molekulskih sond z nanodelci, kar je odprlo še več možnosti za njihovo uporabo. Za opremljanje fluorescenčnih molekul jih moramo najprej podaljšati z ustreznim vmesnikom, da jih lahko potem vežemo na nanodelce. Eden od pristopov je vezava podaljšane fluorescenčne molekule na biotin, kar omogoči njeno vezavo na magnetne nanodelce, opremljene s streptavidinom. V okviru diplomskega dela smo uspešno sintetizirali fluorescenčno molekulo in jo preko etilenglikolnega vmesnika vezali na biotin. Izhodni Boc zaščiteni alkohol smo z estrenjem podaljšali v karboksilno kislino, na katero smo nato vezali fluorescenčno molekulo, ki smo jo predhodno dobili s Henryjevo reakcijo. V naslednjem koraku smo odstranili Boc zaščitno skupino in prosto aminsko skupino vezali na biotin, ki smo ga prej aktivirali s PyBOP. Končne produkte smo ustrezno očistili in okarakterizirali. Fluorescent molecules have played a key role in bioanalytics since the 1950s. In recent years, new approaches for functionalizing fluorescent molecular probes with nanoparticles have been developed, expanding the scope of potential applications. To functionalize fluorescent molecules, we first need to extend them with an appropriate linker before we can attach them to nanoparticles. One approach involves conjugating the elongated fluorescent molecule to biotin, which then allows it to bind to streptavidin-modified magnetic nanoparticles. In this bachelor’s thesis we successfully synthesised a fluorescent molecule and conjugated it to biotin via an ethylene glycol linker. The starting Boc-protected alcohol was elongated into a carboxylic acid through an esterification reaction. Subsequently, a fluorescent probe, obtained by a Henry reaction, was coupled to the carboxylic acid. Afterwards, the Boc protective group was removed and the released free amine linked to biotin, which had been activated with PyBOP prior to the reaction. The final products were purified and characterized. sinteza molekulska sonda biotin etilenglikol synthesis molecular probe biotin ethylene glycol true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-09 09:45:01 2025-09-09 09:45:08 2025-10-30 10:03:44 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25533 255363843 Cebulj_Dasa_-_Podaljsanje_fluorescencne_molekulske_sonde_z_etilenglikolom.pdf Cebulj_Dasa_-_Podaljsanje_fluorescencne_molekulske_sonde_z_etilenglikolom.pdf 1 9C6FD905A20EEB6D14395360270E6A9F 46f84dbcaf2e8ede19d592ec48bc68ccefe94dd862b76c9ce7d566a71ae196b0 c0aafc35-8d50-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=216456 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0