20.500.12556/RUL-172519 In vitro razgradnja hitozanskih filmov s komercialnimi encimi In vitro degradation of chitosan films by commercial enzymes Problem odpadne plastike je eden izmed večjih okoljskih problemov. Proizvodnja plastike le še narašča. Eden izmed možnih rešitev je razvoj novih materialov, sposobnih spontane razgradnje v okolju. Primer takšnega materiala je hitozan, pridobljen iz hitina, ki je osnovni gradnik ogrodja rakov in školjk. Hitozan je skupno ime za skupino hitinov, deacetiliranih do različnih stopenj. Diploma je bila napisana v sklopu projekta Microbeast. Raziskan je bil vpliv komercialnih encimov lakaze, celulaze, lipaze in alkohol dehidrogenaze na razgradnjo pripravljenih hitozanskih filmov v časovnem obdobju 48 h. Dani encimi so bili izbrani zaradi njihove dostopnosti in prisotnosti v prsti. Na podlagi rezultatov imata največji vpliv encima celulaza in lipaza, medtem ko alkohol dehidrogenaza in lakaza nista imela bistvenega vpliva. Hitozan je bil lažje razgradljiv pri pH 6 v primerjavi s pH 7. The problem of plastic waste is one of the biggest environmental problems. Plastic production continues to grow. One possible solution is the development of new materials capable of spontaneous degradation in the environment. An example of such a material is chitosan, obtained from chitin, which is the basic building block of the exoskeleton of crabs and shellfish. Chitosan is the collective name for a group of chitins deacetylated to varying degrees. The thesis was written as part of the Microbeast project. The influence of commercial enzymes Laccase, Cellulase, Lipase, and alcohol dehydrogenase on the degradation of prepared chitosan films over a period of 48 hours was investigated. These enzymes were selected because of their availability and presence in soil. Based on the results, cellulase and lipase had the greatest impact, while alcohol dehydrogenase and laccase had no significant effect. Chitosan was more easily degradable at pH 6 compared to pH 7. encimi biorazgradnja hitozan biopolimer enzymes biodegradation chitosan biopolymer true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-08 09:45:00 2025-09-08 09:45:06 2025-10-29 09:40:33 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25308 255174659 Belak_Anej_-_In_vitro_razgradnja_hitozanskih_filmov_s_komercialnimi_encimi.pdf Belak_Anej_-_In_vitro_razgradnja_hitozanskih_filmov_s_komercialnimi_encimi.pdf 1 EE198DF25669DDD3D84AC77D187CB1B6 bcacf2bd775c0ebf67b9b8a1f54a33ba35d0bc78a8f0d21e7fb0f3b6831ac0a8 952ad738-8c87-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=216347 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0