20.500.12556/RUL-172082 Vpliv zunajceličnega matriksa na izražanje glikogen sintaze in glikogen fosforilaze ter prenašalcev GLUT1 in GLUT4 v kulturi skeletnomišičnih celic. Effect of extracellular matrix on the expression of glycogen synthase and glycogen phosphorylase and transporters GLUT1 and GLUT4 in cultured skeletal muscle cells. Skeletne mišice so kompleksni organi, ki predstavljajo približno 40 % mase človeškega telesa. Omogočajo nam gibanje, pokončno držo, hitre odzive na zunanje dražljaje ter skladiščenje hranil. Za svoje delovanje potrebujejo kemično energijo. Pri sesalcih skeletne mišice predstavljajo glavno mesto za odlaganje glukoze in shranjevanje glikogena za svoje potrebe. Glukoza v skeletne mišice prehaja preko prenašalcev GLUT1 in GLUT4. Kadar je koncentracija glukoze visoka jo glikogen sintaza 1 pretvarja v glikogen, zaradi krčenja pa mišična glikogen fosforilaza glikogen cepi na molekule glukoze. Za adhezijo, proliferacijo, diferenciacijo in migracijo skeletnomišičnih celic je pomembno njihovo okolje. Ključne komponente, potrebne za delovanje skeletnomišičnih celic, želimo priskrbeti tudi celicam, ki jih gojimo in vitro. Primera komercialnih zunajceličnih matriksov sta Matrigel in Geltrex. Namen raziskovalnega dela je bil preveriti vpliv podlage na izražanje dveh encimov, glikogen sintaze 1 in mišične glikogen fosforilaze, ter dveh prenašalcev, GLUT1 in GLUT4. Postavili smo dve hipotezi. V prvi smo predpostavili, da Matrigel in Geltrex povečata izražanje glikogen sintaze 1 (GYS1) in mišične glikogen fosforilaze (PYGM) v primarnih človeških skeletnomišičnih celicah. V drugi pa, da Matrigel in Geltrex povečata izražanje prenašalcev GLUT1 in GLUT4 v primarnih človeških skeletnomišičnih celicah. Rezultati so delno potrdili naši hipotezi. Ugotovili smo, da prihaja do povečanega izražanja encimov glikogen sintaze 1 in mišične glikogen fosforilaze v celicah, ki smo jih nasadili na podlagi Matrigel ali Geltrex, količina mRNA pa se ni razlikovala. Na izražanje prenašalcev GLUT1 in GLUT4 pa podlaga ni imela neposrednega vpliva. Skeletal muscles are a complex organs that represent approximately 40 % of the human body mass. They enable our movement and posture, respond quickly to external stimuli, and store various nutrients. For proper function they require chemical energy. In mammals, skeletal muscles represent the main site for glucose deposition and glycogen storage for their needs. Glucose uptake in skeletal muscles is facilitated through transporters GLUT1 and GLUT4. When the glucose concentration is high, glycogen synthase 1 converts it into glycogen. During contraction muscle glycogen phosphorylase cleaves glycogen into glucose molecules. The environment is important for the adhesion, proliferation, differentiation, and migration of skeletal muscle cells, which is why efforts are made to provide similar conditions for our in vitro models. Our attempt was to provide the key components for the function of cultured primary human skeletal muscle cells. Examples of commercial extracellular matrixes that we used are Matrigel and Geltrex. The aim of our research was to examine the influence different well coatings on the expression of two enzymes, glycogen synthase 1 and muscle glycogen phosphorylase, and two transporters, GLUT1 and GLUT4. We hypothesized that Matrigel and Geltrex would increase the expression of glycogen synthase 1 (GYS1) and muscle glycogen phosphorylase (PYGM) and the transporters GLUT1 and GLUT4 in primary human skeletal muscle cells. The results partially confirmed our hypothesis. We discovered that there was an increased expression of the enzymes glycogen synthase 1 and muscle glycogen phosphorylase in cells plated on Matrigel or Geltrex matrix, but the amount of mRNA did not differ. The matrix did not impact the expression of transporters GLUT1 and GLUT4. skeletnomišične celice glikogen sintaza 1 mišična glikogen fosforilaza GLUT1 GLUT4 zunajcelični matriks skeletal muscle cells glycogen synthase 1 muscle glycogen phosphorylase GLUT1 GLUT4 extracellular matrix true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-05 10:50:00 2025-09-05 10:50:08 2025-10-22 09:57:53 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25676 254219011 Habot_Hanna_-_Vpliv_zunajcelicnega_matriksa_na_izrazanje_glikogen_sintaze_in_glikogen_fosforilaz.pdf Habot_Hanna_-_Vpliv_zunajcelicnega_matriksa_na_izrazanje_glikogen_sintaze_in_glikogen_fosforilaz.pdf 1 039F9E0DD0459158AC125DF3010B7C88 2f3abccaffb94d24ecd0dc405a9a6ef28aa34feb69c1fbcfb28156e56c5e1bd2 2da15567-8a35-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=215820 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0