20.500.12556/RUL-171716 Celične terapije za zdravljenje kostnih poškodb in bolezni Cell therapies for the treatment of bone diseases and injuries V diplomskem delu smo raziskali terapevtski potencial različnih vrst celic pri regeneraciji kostnih poškodb in bolezni. Osredotočili smo se na vrste celic, mehanizme delovanja ter načine aplikacije. Namen diplomskega dela je bil celovit prikaz terapevtskega potenciala različnih celičnih virov, kot so mezenhimske, inducirane pluripotentne, embrionalne in krvnotvorne matične celice ter periostalne celice. Ugotovili smo, da imajo mezenhimske matične celice najširšo klinično uporabo zaradi svoje sposobnosti diferenciacije v osteoblaste, imunomodulatornega delovanja in nizke imunogenosti. Primerjali smo tudi postopke vnosa celic v telo, pri čemer se je uporaba nosilnih materialov (scaffoldov in hidrogelov) izkazala za učinkovitejšo kot injekcijska aplikacija, saj omogoča boljšo retencijo celic in spodbujeno regeneracijo kostnega tkiva. Poleg tega smo izpostavili pomen bioaktivnih molekul, kot so rastni dejavniki in zunajcelični vezikli, ki omogočajo tudi brezcelične terapevtske pristope. Sklepamo, da so celične terapije obetavna dopolnitev klasičnim metodam zdravljenja, zlasti pri kompleksnih poškodbah, kjer tradicionalne metode pogosto ne zadostujejo. Uspešna klinična uporaba bo zahtevala nadaljnje raziskave, standardizacijo in dolgoročne varnostne študije. In this thesis, we investigated the therapeutic potential of various cell types in the regeneration of bone injuries and diseases. We focused on the types of cells used, their mechanisms of action, and the methods of application. The aim of the thesis was to provide a comprehensive overview of the therapeutic potential of different cellular sources, including mesenchymal stem cells, induced pluripotent stem cells, embryonic stem cells, hematopoietic stem cells and periosteal cells. We found that mesenchymal stem cells have the widest clinical application due to their ability to differentiate into osteoblasts, their immunomodulatory effects, and low immunogenicity. We also compared cell delivery methods and found that the use of carrier materials (such as scaffolds and hydrogels) proved more effective than injectable application, as it allows for better cell retention and enhanced regeneration. In addition, we highlighted the importance of bioactive molecules such as growth factors and extracellular vesicles, which enable cell-free therapeutic approaches. We conclude that cell-based therapies represent a promising complement to conventional treatment methods, especially in complex injuries where traditional approaches often fall short. Successful clinical implementation will require further research, standardization, and long-term safety studies. celične terapije regeneracija kosti matične celice tkivno inženirstvo Cell therapies bone regeneration stem cells tissue engineering true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-08-31 07:17:20 2025-08-31 07:17:22 2025-09-17 03:50:21 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 261894 247540227 50789.pdf 50789.pdf 1 9E1BA07625AF130000E9F7C25224412A 87594e7d35367c49d563c1759b99f67c973f8fb02ddd349aaa773456bfb9a5d2 a2105f93-8629-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=215098 Biotehniška fakulteta 0 0 0