20.500.12556/RUL-171707 Sekundarni metaboliti navadne lisičke Secondary metabolites of the golden chanterelle. Zlate lisičke (Cantharellus cibarius) vsebujejo številne metabolite, po navadi so to sekundarni metaboliti oziroma produkti primarnega in sekundarnega metabolizma, ki jih gliva ne potrebuje neposredno za preživetje in razmnoževanje. Za potrebe raziskovanja in gojenja lisičk je bilo izvedenih mnogo poskusov gojenja glive C. cibarius v nadzorovanih in laboratorijskih pogojih. Dosežena je bila rast micelija na trdni kulturi ter redka frutifikacija na mladih, simbiontskih rastlinah, a brez večjih in ponovljivih uspehov gojenja. Cilj diplomske naloge je bil opraviti kratek povzetek pregleda sekundarnih metabolitov glive Cantharellus cibarius ter opisati literaturo o njihovih učinkih, ki so bili analizirani na raznih kulturah bakterijskih in sesalskih celic. Kot sekundarne metabolite lisičk literatura omenja strukturno zelo raznolike spojine, kot redki polisaharidi, polifenolne spojine, steroli, ne-ribosomalni peptidi, pigmenti, terpenoidi, poliketidi in indolne spojine. Še posebej so zanimivi polisaharidi in fenolne spojine, ki imajo zanimive učinke na različne celične kulture in mikroorganizme. Pri bakterijah metaboliti kažejo protimikrobno delovanje, na sesalskih celicah pa protirakasto, citotoksično, antioksidativno in med drugimi tudi regenerativno delovanje. Način delovanja nekaterih metabolitov je pogosto podoben mehanizmom delovanja registriranih zdravil. Zanimivo je tudi protirakasto delovanje siRNA glive C. cibarius, saj ima podobne lastnosti kot polisaharidi in fenolne spojine zlate lisičke. Učinki metabolitov so navadno testirani z ekstrakti polarnih ali nepolarnih topil, ki po ekstrakciji vsebujejo glivne sekundarne metabolite. Bakterijske in sesalske celične kulture se nato izpostavi različnim koncentracijam ekstrakta glive C. cibarius, opazuje se odziv celic na prisotnost metabolitov. Rezultati raziskav so nedvomno zanimivi, vendar so potrebne klinične raziskave za bolj natančno spoznavanje delovanja in uporabnosti metabolitov iz glive C. cibarius. Golden chanterelles (Cantharellus cibarius) contain various metabolites, usually secondary metabolites or in other words products of the primary and secondary metabolic pathways not directly needed for survival and reproduction. Several attempts at cultivating the fungi C. cibarius in controlled laboratory conditions have been attempted. Mycelium growth on petri dishes and frutification on young, symbiotic plant partners are both recorded, but attempts at repeating the findings have been unsuccessfull. The goal of this bachelor thesis was to create a short summary of the overview regarding secondary metabolites of C. cibarius and give commentary on the collected literature regarding the effects of metabolites on various cell cultures both bacterial and mammalian. Studies consider many structurally diverse substances to be secondary metabolites, such as uncommon polysacharides, polyphenols, sterols, non-ribosomal peptides, pigments, terpenoids, polyketides and indole compunds. These metabolites, polysaccharides and phenolic compounds especially, present interesting effects when exposed to various kinds of bacterial and mamalian cell cultures. The metabolites show antimicrobial effects on bacteria and antikarcinogenic, cytotoxic, antioxidant and regenerative effects among others. The effects of certain metabolites of the golden chanterelle are sometimes similar to the effects of registered drugs. The siRNA of C. cibarius is also intriguing, as its effects appear similar to those of secondary metabolites of the chanterelle, such as polysaccharides and polyphenols. Studies usually use polar and nonpolar solvents to achieve sucessfull extraction of metabolites belonging to C. cibarius. Bacterial and mammalian cell cultures are then exposed to the extracts of the chanterelle at various concentrations and the cellular responses are observed. The results of such studies are certainly intriguing but clinical studies are required to show more accurate traits of the C. cibarius metabolites and to shed further light on their possible applications. lisičke metaboliti sekundarni metaboliti učinki golden chanterelle metabolites secondary metabolites aplications true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-08-31 07:16:25 2025-08-31 07:16:30 2025-09-16 03:55:22 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 261885 247323651 50719.pdf 50719.pdf 1 25EA09B7BACF28A1E2C756E2AA50AD4D ebabe5e13d9ace392f66ec3f6a39230f90f980fd5ab0f0401e47228a1e11f373 835c7f8a-8629-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=215089 Biotehniška fakulteta 0 0 0