20.500.12556/RUL-171377 Pridelava in izolacija psilocibina Production and isolation of psilocybin Psilocibin je naravna psihoaktivna spojina, ki jo proizvajajo nekatere vrste gliv, predvsem glive rodu Psilocybe. V zadnjih letih se je zaradi zanimivih terapevtskih učinkov psilocibina, zlasti pri zdravljenju depresije, ponovno vzbudilo zanimanje za ta sekundarni metabolit. Namen te naloge je bil pripraviti kratek pregled kemijskih in fizikalnih lastnosti psilocibina, njegovo biosintezo, terapevtski potencial, ter možnosti njegove biosinteze z uporabo gensko spremenjenih mikroorganizmov. Primerjane so različne metode pridelave kot so kemijska sinteza, encimska sinteza (biotransformacija) in biosinteza. Prav tako so opisani postopki izolacije psilocibina iz bioprocesne brozge. Za sintezo je bilo predstavljenih več organizmov, podrobneje pa je predstavljena biosinteza z bakterijo Escherichia coli. V okviru naloge so bile identificirane ključne prednosti biosinteze, kot so možnost prenosa procesa v večje merilo, visoki titri in prilagodljivost sistema. Trenutno najvišji titri psilocibina doseženi z biosintezo segajo do 1,46 g/L, pri čemer so prisotni uporabljeni intermediati psilocibina, kot začetni substrat, brez katerih so proizvedeni titri zelo nizki. Pridelavaa psilocibina »de novo« s prokarionti je možna, vendar potrebuje veliko izboljšav, da bi bila primerna za industrijsko pridelavo psilocibina. Na podlagi analizirane literature lahko zaključimo, da je biotehnološka pridelava psilocibina izvedljiva, vendar zahteva nadaljnje izboljšave za industrijsko pridelavo za farmacevtsko uporabo. Psilocybin is a naturally occurring psychoactive compound produced by certain species of fungi, primarily those of the Psilocybe genus. In recent years, due to psilocybin’s promising therapeutic effects—particularly in the treatment of depression—scientific interest in the compound has been reignited. The purpose of this thesis was to review chemical and physical properties of psilocybin, its biosynthesis, therapeutic potential, and the possibilities of its production using genetically modified microorganisms. Various production methods were reviewed, including chemical synthesis, enzymatic synthesis (biotransformation), and biosynthesis. The work also describes procedures for isolating psilocybin from bioprocess broth. Several production organisms were introduced, with a more detailed focus on biosynthesis using the bacterium Escherichia coli. The thesis identified key advantages of biosynthesis, such as scalability, high titers, and system flexibility. Currently, the highest psilocybin titers achieved via biosynthesis reach up to 1.46 g/L, this type of process involves the use of intermediates; without them, production titers remain very low. »De novo« production of psilocybin in prokaryotes is possible, but requires substantial optimization to become suitable for industrial-scale manufacturing. Based on the reviewed literature, the thesis confirms that biotechnological production of psilocybin is feasible, but further improvements are necessary to enable its pharmaceutical-scale application. Psilocibin psilocin pridelava biosinteza mikroorganizmi izolacija psilocybin psilocin production biosnythesis microorganisms isolation true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-08-24 07:15:42 2025-08-24 07:15:50 2025-08-31 03:53:55 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 261383 246471427 50551.pdf 50551.pdf 1 772ED58DAA78E92B9EE64A31B2686EAF 5d67586c899ebb8f232f208fe5cb84a1f272fa030a07f86d1584d7c27e8b28a4 48b0faeb-80a9-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214664 Biotehniška fakulteta 0 0 0