20.500.12556/RUL-171328 Effect of non-thermal treatments of clear apple juice on exogenous pectinases Pulsed electric field (PEF) and high-pressure processing (HPP) are non-thermal treatments, developed to ensure preservation of food products whilst maintaining taste and valuable nutrients. In this study, we investigated their potential for the inactivation of 3 commercial exogenous pectinases (polygalacturonase, pectin transeliminase, pectin esterase) commonly used in juice processing for clarification of juices. The inactivation of these enzymes after processing is mandatory by European law. Clear apple juice was treated with both nonthermal processing methods, as well as with thermal pasteurization as the standard method. For HPP, 3 pressures (250, 450, and 600 MPa) and different holding times (from 2 to 12 min) were tested. For PEF, 3 electric field intensities (10, 13, and 15 kV/cm) and different specific energy values (from 121 to 417 kJ/kg). Standard thermal pasteurization resulted in a complete inactivation of all tested pectinases. HPP treatment only showed marginal effects on polygalacturonase and pectin transeliminase at the highest pressure and holding times, which are beyond levels used in industrial settings. For PEF, dependence upon high electric field strength and specific energy values was evident; however, here too, the effect was only moderate at the levels attainable within the scope of this study. Assuming a continued linear relationship, usable results could be achieved in an industrial setting, albeit under more extreme conditions. enzyme inactivation pectinase fruit juice HPP PEF thermal pasteurization non-thermal treatment exogenous enzyme inaktivacija encimov pektinaze sadni sokovi HPP PEF termalna pasterizacija netermične metode netermična obdelava encimi eksogenost true false true Angleški jezik Slovenski jezik Članek v reviji 2025-08-22 14:00:02 2025-08-22 14:00:08 2025-08-26 03:29:13 0000-00-00 00:00:00 2025 0 0 15 str. iss. 4, art. 113 Vol. 11 Aug. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.7 2306-5710 10.3390/beverages11040113 245279747 Effect_of_Non-Thermal_Treatments_of_Clear_Apple_Juice_on_Exogenous_Pectinases.pdf Effect_of_Non-Thermal_Treatments_of_Clear_Apple_Juice_on_Exogenous_Pectinases.pdf 1 70F10FF089FADD73B7233BD37C9D8FD3 2519fcbc5a30103673afb798be19a2bd6f2f5c0c80c1b6edfd070598ec0e4826 c9d629f5-7f4f-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214579 https://www.mdpi.com/2306-5710/11/4/113 1 725c4f27-7f4f-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214578 Biotehniška fakulteta 0 0 0