20.500.12556/RUL-171277 Vrstno specifična absorpcija bakra in cinka v trosnjake saprofitskih gliv Species-specific absorption of copper and zinc to fruiting bodies of saprophytic fungi Analizirali smo vsebnost kovin v trosnjakih gliv: Agaricus bitorquis, Agaricus bisporus, Armillaria mellea, Hericium erinaceus, Laetiporus sulphureus, Lentinula edodes, Macrolepiota procera, Kuehneromyces mutabilis, Pleurotus eryngii in Pleurotus ostreatus. Vzorce smo najprej posušili, nato zmleli in stistnili v tablete, primerne za analizo XRF. Primerjali smo vsebnosti kovin med posameznimi vrstami gliv. Pri nabranih in laboratorijsko vzgojenih glivah smo s pomočjo analize XRF preučevali povezave med koncentracijami kovin v substratu in njihovimi vrednostmi v trosnjakih. Pri kupljenih gobah smo vsebnost kovin primerjali z ostalimi skupinami vzorcev. V laboratorijskih pogojih smo gojili Hericium erinaceus in Pleurotus ostreatus na substratih z dodatkom znanih koncentracij Cu in Zn. Na podlagi rezultatov smo opisali korelacije med vsebnostjo kovin v substratu in v trosnjakih ter opazovali vpliv visokih vnosov Cu in Zn. Za obe gojeni vrsti smo izračunali BCF, ki nam je omogočil boljši vpogled v akumulacijske sposobnosti gliv za Cu in Zn. Povprečne vrednosti BCF so se zniževale z naraščanjem koncentracij dodanih kovin, kar kaže na regulacijo absorpcije s strani gliv in prisotnost zgornje meje sprejema kovin. Rezultati potrjujejo, da je absorpcija kovin pri glivah omejena in ni linearno naraščajoča. Dodatno smo s presejalnim testom na PDA gojišču, z znanimi koncentracijami Zn, analizirali inhibitorni učinek cinka na rast Hericium erinaceus. The metal content of Agaricus bitorquis, Agaricus bisporus, Armillaria mellea, Hericium erinaceus, Laetiporus sulphureus, Lentinula edodes, Macrolepiota procera, Kuehneromyces mutabilis, Pleurotus eryngii and Pleurotus ostreatus was analysed. The samples were first dried, then ground and pressed into tablets suitable for XRF analysis. The metal contents were compared between the different fungal species. For harvested and laboratory-grown mushrooms, XRF analysis was used to investigate the relationships between the metal concentrations in the substrate and in the substrate. The metal content of purchased mushrooms was compared with the other sample groups. Hericium erinaceus and Pleurotus ostreatus were grown under laboratory conditions on substrates with known concentrations of Cu and Zn. The results were used to describe the correlations between the metal content in the substrate and in the substrate and to observe the effects of high Cu and Zn additions. For both cultivated species, we calculated the BCF, which gave us a better insight into the accumulation capacity of the fungi for Cu and Zn. The average BCF values decreased with increasing concentration of the added metals, indicating a regulation of uptake by the fungi and the existence of an upper limit for metal uptake. The results confirm that the uptake of metals by fungi is limited and does not increase linearly. Furthermore, the inhibitory effect of zinc on the growth of Hericium erinaceus was analysed by a screening test on PDA medium with known Zn concentrations. glive kovine užitne gobe saprofiti bioakumulacija kovin fungi metals edible mushrooms saprophytes bioaccumulation of metals true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-08-22 07:15:29 2025-08-22 07:15:37 2025-08-27 03:34:19 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 261155 246324483 49605.pdf 49605.pdf 1 26F3489185134074613D7767779B27F9 1b245a4c641a2a3d97bc40fa5b3448d278f54618477d2921af6c5aab45a40fc5 eeadde64-7f16-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214498 Biotehniška fakulteta 0 0 0