20.500.12556/RUL-171186 The equivalence of different types of electric pulses for electrochemotherapy with cisplatin − an in vitro study Enakovrednost različnih vrst električnih pulzov za elektrokemoterapijo s cisplatinom Background Electrochemotherapy (ECT) is a treatment involving the administration of chemotherapeutics drugs followed by the application of 8 square monopolar pulses of 100 μs duration at a repetition frequency of 1 Hz or 5000 Hz. However, there is increasing interest in using alternative types of pulses for ECT. The use of high-frequency short bipolar pulses has been shown to mitigate pain and muscle contractions. Conversely, the use of millisecond pulses is interesting when combining ECT with gene electrotransfer for the uptake of DNA-encoding proteins that stimulate the immune response with the aim of converting ECT from a local to systemic treatment. Therefore, the aim of this study was to investigate how alternative types of pulses affect the efficiency of the ECT. Materials and methods We performed in vitro experiments, exposing Chinese hamster ovary (CHO) cells to conventional ECT pulses, high-frequency bipolar pulses, and millisecond pulses in the presence of different concentrations of cisplatin. We determined cisplatin uptake by inductively coupled plasma mass spectrometry and cisplatin cytotoxicity by the clonogenic assay. Results We observed that the three tested types of pulses potentiate the uptake and cytotoxicity of cisplatin in an equivalent manner, provided that the electric field is properly adjusted for each pulse type. Furthermore, we quantified that the number of cisplatin molecules, resulting in the eradication of most cells, was 2−7 × 107 per cell. Conclusions High-frequency bipolar pulses and millisecond pulses can potentially be used in ECT to reduce pain and muscle contraction and increase the effect of the immune response in combination with gene electrotransfer, respectively. electrochemotherapy electroporation cisplatin uptake phenomenological model equivalent puls parameters elektrokemoterapija elektroporacija vnos cisplatina fenomenološki model ekvivalentnost parametrov pulzov true false true Angleški jezik Slovenski jezik Članek v reviji 2025-08-18 13:59:30 2025-08-18 13:59:36 2025-08-19 04:11:36 0000-00-00 00:00:00 2024 0 0 Str. 51-66, VI iss. 1 Vol. 58 Mar. 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 602.621 1318-2099 10.2478/raon-2024-0005 186310403 The-equivalence-of-different-types-of-electric-pulses-for-electrochemotherapy-with-cisplatin-−-an-in-vitro-study.pdf The-equivalence-of-different-types-of-electric-pulses-for-electrochemotherapy-with-cisplatin-−-an-in-vitro-study.pdf 1 7F51276E2A08E47FA5F83A4C4C31459A c5afe0cf2f5e38a298221b77649178d684f8cfa3bbfef4a5c4521286a62be0ca 043445f8-7c2b-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214252 https://sciendo.com/article/10.2478/raon-2024-0005 1 b8394cb4-7c2a-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=214251 Fakulteta za elektrotehniko 0 0 0