20.500.12556/RUL-171120 Comparative analysis of enzymatic activity, phenolic and ascorbic acid stability and browning dynamic in white and red-fleshed apple (Malus domestica Borkh.) cultivars post-air exposure This study investigates enzymatic browning in several white- and red-fleshed apple cultivars by analyzing polyphenol oxidase (PPO) and peroxidase (POX) activities, total phenolic content (TPC), anthocyanins, ascorbic acid, and color changes during air exposure. Six white-fleshed cultivars (‘Topaz’, ‘Opal’, ‘Red Boskoop’, ‘Golden Delicious’, ‘Crown Prince Rudolph’, ‘Idared’) and the red-fleshed cultivar ‘Baya Marisa’ were evaluated. Results revealed cultivar-specific enzymatic activity influencing browning susceptibility. ‘Idared’ exhibited the highest PPO activity and the strongest browning potential, while ‘Baya Marisa’ showed low PPO but unexpectedly high POX activity. All white-fleshed cultivars were prone to browning, as indicated by a significant increase in browning index (BI) from 0 to 480 min. In contrast, ‘Baya Marisa’ showed minimal color change and no significant BI variation, suggesting limited browning. However, due to high a* values, BI may not be suitable for assessing browning in red-fleshed cultivars. ‘Golden Delicious’ had the highest initial ascorbic acid concentration and the slowest degradation rate. ‘Baya Marisa’ consistently exhibited the highest TPC, attributed to elevated anthocyanin levels. These findings emphasize the importance of cultivar selection in managing browning and highlight limitations of BI as a universal indicator, particularly for red-fleshed apples. enzyme activity polyphenol oxidase peroxidase phenolic content browning index time degradation encimska aktivnost polifenol-oksidaza peroksidaza vsebnost fenolov degradacija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-08-06 14:08:03 2025-08-06 14:08:10 2026-06-18 04:33:50 0000-00-00 00:00:00 2025 0 0 9 str. art. 108040 Vol. 147 Nov. 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 634.11 0889-1575 10.1016/j.jfca.2025.108040 243597827 1-s2.0-S0889157525008555-main.pdf 1-s2.0-S0889157525008555-main.pdf 1 DC80BEAB0BAE83356DE69C04D8234E76 56c783055d7260eea126eb9e8e3628a23acaec825ab3d0edd11bf9b11dae5c76 2249b908-6a26-11f1-9b0d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=235827 https://www.sciencedirect.com/science/article/pii/S0889157525008555 1 f6013dd8-72bd-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=213990 Biotehniška fakulteta 0 0 0