20.500.12556/RUL-170840 Analiza cirkulirajočih tumorskih celic v krvi bolnic s seroznim rakom jajčnikov visokega gradusa Circulating tumor cells in the blood of patients with high-grade serous ovarian carcinoma Rak jajčnikov je najagresivnejša oblika ginekološkega raka, saj se uvršča med najpogostejše vzroke smrti zaradi raka pri ženskah, tako globalno kot tudi v Sloveniji. V magistrskem delu smo s tehnologijo Parsortix iz periferne krvi dvajsetih bolnic z diagnosticiranim seroznim rakom jajčnikov visokega gradusa (HGSC), stadijev FIGO III in IV, uspešno izolirali cirkulirajoče tumorske celice (CTC). Glavni cilj raziskave je bil morfološko ovrednotiti CTC in jih povezati s klinično-patološkimi značilnostmi bolezni. Z uporabo imunocitokemije in imunofluorescence smo potrdili njihovo izrazito heterogenost, kar odraža kompleksnost biološkega vedenja CTC ter njihovo potencialno vlogo pri metastaziranju. Ugotovili smo statistično značilno povezavo med številom CTC in številom njihovih skupkov, kar nakazuje, da so skupki lahko pomemben indikator tumorske aktivnosti. Med stadijema FIGO III in IV sicer ni bilo statistično značilnih razlik v mediani števila CTC, vendar podatki kažejo na trend višjih vrednosti pri FIGO IV. Statistično značilne razlike v velikosti jedra, površini in obsegu CTC med stadiji dodatno potrjujejo morfološko heterogenost celic glede na napredovalost bolezni. Ovarian cancer is the most aggressive form of gynecological malignancy and ranks among the leading causes of cancer-related death in women, both globally and in Slovenia. In this master’s thesis, we successfully isolated circulating tumor cells (CTCs) from the peripheral blood of twenty patients diagnosed with high-grade serous ovarian cancer (HGSC), FIGO stages III and IV, using the Parsortix technology. The main objective of the study was to perform a morphological evaluation of CTCs and to assess their correlation with clinicopathological features of the disease. Through immunocytochemistry and immunofluorescence, we confirmed pronounced heterogeneity among CTCs, reflecting the biological complexity of these cells and their potential role in metastasis. We identified a statistically significant correlation between the number of CTCs and the number of CTC clusters, suggesting that clustering may be an important indicator of tumor activity. Although there were no statistically significant differences in the median CTC count between FIGO stage III and IV patients, the data indicated a trend toward higher values in stage IV. Statistically significant differences in nuclear size, surface area, and perimeter of CTCs across FIGO stages further support the presence of morphological heterogeneity associated with disease progression. Serozni rak jajčnikov visokega gradusa HGSC cirkulirajoče tumorske celice CTC Parsortix metoda morfološka analiza High-grade serous ovarian carcinoma HGSC circulating tumor cells CTCs Parsortix method morphological analysis true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-07-18 07:15:13 2025-07-18 07:15:24 2025-07-20 03:43:29 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 257837 243080707 49489.pdf 49489.pdf 1 E902267AF668517804A6B9134CC23472 705e30aa0a9a437cd44eebc5f37c7b2274ebdf58b70e593d734c302ed14512a0 39cb2ecd-6396-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=213099 Biotehniška fakulteta 0 0 0