20.500.12556/RUL-170808 Pomen obstojnosti virusa lisavosti in mozaika paradižnika v vodi za širjenje okužbe Tomato mottle mosaic virus in aqueous environment - survival and importance of water mediated transmission Virus lisavosti in mozaika paradižnika (ToMMV) je stabilen tobamovirus, ki lahko povzroča gospodarsko škodo v pridelavi paradižnika in paprike. Tobamovirusi se prenašajo z okuženimi semeni, sadikami in mehansko. Nekateri se lahko širijo tudi z vodo, vendar za ToMMV ta način še ni bil raziskan. V tej nalogi smo preverjali, ali se ToMMV lahko sprošča iz korenin okuženih rastlin v hranilno raztopino, preživi v vodi in povzroči okužbo zdravih rastlin ob uporabi z virusom kontaminirane vode ali substrata. Glavne ugotovitve te raziskave so bile, da se infektivni delci ToMMV sproščajo iz korenin okuženih rastlin v hranilno raztopino, kjer ostanejo prisotni več tednov. Virus je v vodi pri sobni temperaturi ohranil infektivnost do 27 tednov. V hidroponskih sistemih je do okužbe zdravih rastlin prišlo le, če so bile njihove korenine mehansko ranjene in so bile izpostavljene hranilni raztopini, kontaminirani s ToMMV. V rastnem substratu je bila okužba potrjena pri sadikah in pri rastlinah, ki so zrasle iz semen, če so bile zalivane z vodo, kontaminirano s ToMMV. Okužba se je pojavila tudi pri rastlinah, posajenih v že kontaminiran substrat, čeprav je bila za zalivanje uporabljena nekontaminirana voda. Rezultati potrjujejo, da je voda lahko pomemben dejavnik pri širjenju ToMMV, kar ima pomembne posledice za upravljanje bolezni v intenzivni pridelavi. Priporočamo uvedbo monitoringa namakalnih vod in substratov ter izvajanje ustreznih higienskih ukrepov za preprečevanje širjenja okužb. Tomato mottle mosaic virus (ToMMV) is a stable tobamovirus that can cause economic damage in tomato and pepper production. Tobamoviruses are primarily transmitted through infected seeds, planting material, and mechanical means, though some can also spread via water. However, for ToMMV, this transmission route has not yet been investigated. In this study, we examined whether ToMMV can be released from infected plants into the nutrient solution, survive in water, and cause infections in healthy plants when contaminated water or substrate is used. The main findings of this research were that infectious ToMMV particles are released from the roots of infected plants into the nutrient solution, where they remain present for several weeks. The virus retained its infectivity in water at room temperature for up to 27 weeks. In hydroponic systems, infection of healthy plants occurred only if their roots were mechanically damaged and exposed to ToMMV-contaminated nutrient solution. In the growth substrate, infection was confirmed in seedlings and plants grown from seeds if they were irrigated with ToMMV-contaminated water. Infection also occurred in plants planted in pre-contaminated substrate, even when uncontaminated water was used for irrigation. The results confirm that water can be a significant factor in the spread of ToMMV, which has important implications for disease management in intensive production. We recommend implementing monitoring of irrigation water and substrates, as well as applying appropriate hygiene measures to prevent the spread of infections. virus ToMMV širjenje z vodo preživetje v vodi hidroponija virus ToMMV transmission by water hydroponic true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-07-17 07:15:06 2025-07-17 07:15:18 2025-07-19 03:40:20 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 257826 242867203 49487.pdf 49487.pdf 1 86CDFF04495A2940C726CAF828E43919 430ea9c76b9c50516297ed861a68cf3723bfdd5081be9c9b193299d52cd96094 15f24d0b-62cb-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=213017 Biotehniška fakulteta 0 0 0