20.500.12556/RUL-170502 Enhancing the outcomes of temporalis fascia tympanoplasty using autologous platelet-rich plasma and gel a randomized controlled trial This study aimed to investigate the impact of platelet-rich plasma (PRP) and platelet-rich gel (PRG) on tympanic membrane closure rates, hearing improvement, and quality of life following tympanoplasty. Methods: Seventy-two patients with chronic tympanic membrane perforations were enrolled in a double-blinded, randomized controlled trial at a single tertiary referral center. All patients underwent tympanoplasty using a temporalis fascia graft and were randomly assigned to one of two groups: one group received standard tympanoplasty alone, while the other received intraoperative application of autologous PRP and PRG, in addition to the standard procedure. Results: The PRP group demonstrated a significantly higher rate of complete tympanic membrane closure compared to the control group (32/36; 88.9% vs. 24/36; 66.7%; p < 0.05). Bone conduction hearing remained unchanged in both groups, while air conduction hearing improved significantly from pre- to post-treatment in each group. However, the difference in air conduction improvement between the PRP group and the control group was not statistically significant (PRP group: Mdn = −8.25; control group: Mdn = −12.20; U = 618; z = −0.54; p = 0.30). Quality of life improved in both the PRP and control groups; however, the difference between the groups was not statistically significant (PRP group: 10.44 ± 10.46; control group: 10.47 ± 8.22; 95% CI [−4.45; 4.40]; t(66) = −0.01; p = 0.16). Conclusions: Our findings suggest that intraoperative application of autologous PRP and PRG may improve tympanoplasty outcomes, particularly in cases with lower expected success rates or when performing minimally invasive transcanal procedures under local anesthesia. However, variability in PRP preparation, application methods, and graft materials across studies limits direct comparisons. Standardized protocols and further controlled studies are necessary to clarify PRP’s clinical value in tympanoplasty. tympanoplasty platelet-rich plasma (PRP) platelet-rich gel (PRG) extracellular vesicles tympanic membrane perforation cartilage graft hearing improvement quality of life randomized controlled trial otologic surgery timpanoplastika plazma bogata s trombociti (PRP) gel bogat s trombociti (PRG) zunajcelični vezikli perforacija bobniča presadek hrustanca izboljšanje sluha kakovost življenja randomizirana kontrolirana študija otološka kirurgija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-07-07 13:27:06 2025-07-07 13:27:09 2025-07-08 04:20:22 0000-00-00 00:00:00 2025 0 0 Str. 1-14 iss. 6, art. 233 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2025-01-01 616.28:544.77.022.533 2075-4426 10.3390/jpm15060233 239140099 jpm-15-00233-v2.pdf jpm-15-00233-v2.pdf 1 13F37589E8DB9A9DA02EFE4DDDA7678B 56c2fc11f34a493d89d9699414addc644571f00f8c906672eca4768e8c317298 c5b02099-5b23-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=212518 https://www.mdpi.com/2075-4426/15/6/233 1 692d3402-5b23-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=212517 Zdravstvena fakulteta Medicinska fakulteta Filozofska fakulteta 0 0 0