20.500.12556/RUL-170444 Ciljno utišanje genov pri glivah z uporabo molekul dsRNA proizvedenih v bakterijskih celicah Targeted gene silencing in fungi using dsRNA molecules produced in bacterial cells Glivni patogeni predstavljajo veliko nevarnost za zdravje ljudi in rastlin. Tradicionalno se jih zatira s fungocidi, ki pa imajo negativne učinke na okolje in zdravje. Ciljno utišanje genov z uporabo eksogeno proizvedene dvoverižne RNA (dsRNA) je obetavna alternativa, saj je njihovo delovanje zelo specifično. Glavna ovira za širšo uporabo je draga proizvodnja dsRNA. Rešitev je produkcija v bakterijah, največkrat uporabljena je Escherichia coli HT115(DE3), alternativa so tudi simbiotske bakterije rastlin, ki jih glive okužujejo. Za dostavo dsRNA molekul se lahko uporabijo žive bakterije, bakterijski lizati ali izolirane dsRNA molekule. Z uporabo živih bakterij lahko zagotovimo zaščito dsRNA pred fizičnimi in biološkimi dejavniki, vendar je treba pri tem zagotoviti varnost in preprečiti pobeg bakterij v okolje. Fungal pathogens pose a major threat to both human and plant health. Traditionally, they are controlled using fungicides; however, these have adverse environmental and health effects. Targeted gene silencing through the use of exogenously produced double-stranded RNA (dsRNA) represents a promising alternative due to its high specificity. The primary obstacle to broader application is the high cost of dsRNA production. Bacterially produced dsRNA could offer a solution. Escherichia coli HT115(DE3) is most commonly used, although symbiotic, plant-associated bacteria inhabiting hosts susceptible to fungal infection are also being explored. For dsRNA delivery, live bacteria, bacterial lysates, or purified dsRNA molecules can be employed. The use of live bacteria offers protection of dsRNA against physical and biological degradation; however, strict biosafety measures are necessary to prevent environmental release of these bacteria. interferenca z RNA glive bakterijska proizvodnja dsRNA Escherichia coli HT115(DE3) fitopatogeni RNA interference fungi bacterial production of dsRNA Escherichia coli HT115(DE3) phytopathogens true false true Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-07-06 07:15:46 2025-07-06 07:15:53 2025-07-07 09:20:09 0000-00-00 00:00:00 2025 0 0 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 257736 241562371 49420.pdf 49420.pdf 1 E1E1D6D13E1CB540CA7EB8493DFFF7DF 0847ed3dd84b3a8d890e3b668420fb0be658f4bf235e69f8b0447c76b18b35fc 60c74751-5a26-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=212448 Biotehniška fakulteta 0 0 0