20.500.12556/RUL-170123 Sposobnost preživetja in tvorbe kolonij sinovijskih primarnih celic, pridobljenih iz kadavrov, po daljšem obdobju njihovega shranjevanja v tekočem dušiku Survival and colony formation ability of synovium-derived primary cells obtained from post mortem donors after their prolonged cryopreservation in liquid nitrogen Mezenhimske matične/stromalne celice (MSC) imajo velik potencial v regenerativni medicini zaradi svoje sposobnosti imunomodulacije in regeneracije poškodovanih tkiv, zlasti pri obnovi hrustanca. Za njihovo dolgoročno shranjevanje in kasnejšo terapevtsko uporabo je ključnega pomena krioprezervacija. Vendar pa ostaja vpliv dolgotrajne hrambe v tekočem dušiku – zlasti večletne – na njihovo življenjsko sposobnost in funkcionalni potencial slabo raziskan. Namen te študije je bil oceniti, ali dolgotrajna krioprezervacija vpliva na življenjsko sposobnost in sposobnost tvorbe kolonij primarnih MSC celic sinovijskega izvora, izoliranih iz postmortalnih darovalcev. Skupno je bilo analiziranih 22 vzorcev (21 iz sinovije in eden iz kostnega mozga, ki je služil kot kontrola), ki so bili po obdobju shranjevanja od dveh do več kot petih let odtaljeni in gojeni in vitro. Njihova proliferativna sposobnost je bila ocenjena s pomočjo testa tvorbe fibroblastnih kolonij (CFU-F). Le pet od 22 vzorcev je po dolgotrajnem shranjevanju uspešno tvorilo kolonije. Statistična analiza ni pokazala pomembne korelacije med trajanjem shranjevanja in sposobnostjo tvorbe kolonij (Spearmanov r = –0,1225, p = 0,5528). Prav tako ni bilo ugotovljene pomembne razlike v trajanju shranjevanja med uspešno in neuspešno krioprezerviranimi vzorci (p = 0,157, Mann-Whitneyjev U-test). Čeprav nizka uspešnost kaže na možen negativen vpliv dolgotrajnega shranjevanja na preživetje MSC, rezultati nakazujejo, da sama dolžina shranjevanja ni odločilen dejavnik. Namesto tega imajo večjo vlogo spremenljivke, kot so biološka variabilnost darovalcev, postmortalno stanje tkiva in ravnanje z vzorci. Mesenchymal stem/stromal cells (MSCs) hold great promise in regenerative medicine due to their ability to modulate immune responses and regenerate damaged tissues, particularly in cartilage repair. Cryopreservation is essential for their long-term storage and future therapeutic use. However, the effects of prolonged cryopreservation—especially when cells are stored for several years in liquid nitrogen—on their viability and functional potential remain poorly understood. This study aimed to assess whether extended cryopreservation impacts the viability and colony-forming ability of synovium-derived primary MSCs isolated from post-mortem donors. A total of 22 primary cell samples (21 synovium-derived and one bone marrow-derived, used as a reference) were thawed after being cryopreserved for periods ranging from two to over five years. Following thawing and in vitro culture, the colony-forming unit fibroblast (CFU-F) assay was used to evaluate their proliferative potential. Out of 22 samples, only five successfully formed colonies. Statistical analysis revealed no significant correlation between storage duration and colony-forming capacity (Spearman's r = –0.1225, p = 0.5528). Similarly, no statistically significant difference in storage times was found between successfully and unsuccessfully cryopreserved samples (p = 0.157, Mann-Whitney U test). While the low recovery rate suggests that prolonged cryopreservation may negatively affect MSC viability, the findings indicate that storage time alone is not a determining factor. Instead, donor variability, post-mortem tissue conditions, and handling procedures likely play a more critical role in cryopreservation outcomes. mezenhimske matične/ stromalne celice sinovijska ovojnica krioprezervacija sposobnost tvorbe kolonij preživetje celic regenerativna medicina mesenchymal stem cells synovium cryopreservation colony forming unit assay cell viability regenerative medicine true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-07-02 13:17:35 2025-07-02 13:17:37 2025-07-03 04:43:17 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 1970-01-01 114969 27032.pdf 27032.pdf 1 5271CE3CA00B6669BF0894175B8904F2 185dcc432786c2f687da208fa71d02294be6214ede294675523a0df0e6d17033 43c96f6c-5734-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=212054 Fakulteta za farmacijo 0 0 0