20.500.12556/RUL-169993 Standard operating procedure for next generation sequencing of RNA isolated form extracellular vesicles within the nanostructurome methods pipeline The standard operating procedure (SOP) for next generation sequencing (NGS) of RNA from extracellular vesicles is presented. The SOP enables purification of small or total RNAs from isolated extracellular vesicles. Next, the SOP describes the sequencing of RNAs using NGS sequencing platform IonTorrent. In the presented protocol we define all the steps from isolation of RNA to sequencing data management, together with the cautions and checkpoints crucial for obtaining reliable high-quality sequencing reads, suitable for further analysis. NGS enables high-throughput DNA and RNA sequencing, offering accuracy, scalability, and cost-efficiency. RNA sequencing is widely used for analysing gene expression, alternative splicing, and non-coding RNAs. NGS also facilitates the study of extracellular vesicles (EVs), which play a key role in intercellular communication, especially in plant-pathogen interactions. Insights gained could inform novel disease management strategies, such as Spray-Induced Gene Silencing, for improved crop protection. next generation sequencing extracellular vesicles RNA-seq total RNA small RNA IonTorrent true false true Angleški jezik Ni določen Znanstveno delo 2025-07-01 13:22:24 2025-07-01 13:22:25 2025-07-02 04:07:20 0000-00-00 00:00:00 2025 0 0 Str. 18-24 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 10.55295/PSL.12.2025.III3 240973827 237747715 Standard+Operating+Procedure+for+Next+Generation+Sequencing+of+RNA+Isolated+form+Extracellular+Vesicles+within+the+Nanostructurome+Methods+Pipeline.pdf Standard+Operating+Procedure+for+Next+Generation+Sequencing+of+RNA+Isolated+form+Extracellular+Vesicles+within+the+Nanostructurome+Methods+Pipeline.pdf 1 E22D37E260FB831D365151787C67F573 53ce47106997f75c7eac354be6ce31701abf28e9803619bbda38cdd6173e586a 05da25b4-566c-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211891 https://ebooks.uni-lj.si/ZalozbaUL/catalog/book/767/chapter/4183 1 c5e69496-566b-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211890 Zdravstvena fakulteta Biotehniška fakulteta 0 0 0