20.500.12556/RUL-169934 Species-specific chemotactic responses of entomopathogenic and slug-parasitic nematodes to cannabinoids from Cannabis sativa L. The increasing environmental and health concerns associated with synthetic pesticides underscore the need for sustainable alternatives in pest management. This study investigates the chemotactic responses of five nematode species—Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, Phasmarhabditis papillosa, and Oscheius myriophilus—to three major cannabinoids from Cannabis sativa: ∆$^9$-tetrahydrocannabinol (THC), cannabigerol (CBG), and cannabidiol (CBD). Using a standardized chemotaxis assay, we quantified infective juvenile movement and calculated Chemotaxis Index (CI) values across varying cannabinoid concentrations. Our results revealed strong species-specific and dose-dependent responses. THC and CBG elicited significant attractant effects in P. papillosa, S. feltiae, and H. bacteriophora, with CI values ≥ 0.2, indicating their potential as behavioral modulators. In contrast, CBD had weaker or repellent effects, particularly at higher concentrations. O. myriophilus exhibited no consistent response, underscoring species-specific variation in chemosensory sensitivity. These findings demonstrate the potential utility of cannabinoids, especially THC and CBG, as biocompatible cues to enhance the efficacy of nematode-based biological control agents in integrated pest management (IPM). Further field-based studies are recommended to validate these results under realistic agricultural conditions. biological control chemosensory sensitivity cannabinoids cannabidiol cannabigerol chemotaxis entomopathogenic nematodes integrated pest management slug-parasitic nematodes tetrahydrocannabinol biotično varstvo rastlin kanabinoidi kanabidiol kanabigerol kemotaksija entomopatogene ogorčice integrirano varstvo rastlin varstvo rastlin tetrahidrokanabinol true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-19 11:27:40 2025-06-19 11:27:46 2025-06-20 04:16:38 0000-00-00 00:00:00 2025 0 0 14 str. iss. 6, art. 1469 Vol. 15 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 632.9 2073-4395 10.3390/agronomy15061469 239923203 agronomy-15-01469.pdf agronomy-15-01469.pdf 1 957371C306F5ED5D4D5E794AB865B293 b2a97d611ad8d1d3c3f5405cbef20e6f12ccbc9cea0a71d1649b1b98ec8d5c2f 8a80df0d-4cee-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211743 https://www.mdpi.com/2073-4395/15/6/1469 1 1d068c5f-4cee-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211742 Biotehniška fakulteta 0 0 0