20.500.12556/RUL-169915 Spelt grass juice : phytochemicals and antiproliferative activity Spelt is gaining popularity due to its nutritional and ecological benefits, particularly in organic farming. Juice from young spelt grass is rich in potassium, phosphorus, manganese, and chromium, along with beneficial pigments and phenolic compounds, all of which support human health. This study examines the phytochemical composition (photopigments and phenolics) determined by visible spectrophotometry and liquid chromatography, as well as the antiproliferative effects of spelt grass juice extracts assessed by the MTT assay. It also explores their selectivity towards human malignant cells (lung A-549 and cervical HeLa) and their potential synergy with doxorubicin, an anticancer drug. Spelt grass juice extracts, particularly aqueous and methanol–water extracts, contained significant amounts of ferulic acid and its derivatives. The methanol–water extracts were similar to aqueous extracts in terms of total phenolics (3464–3601 µg/g DW), while the acetone–water extracts had a significantly lower content (around 2700 µg/g DW). The antiproliferative effect of spelt extracts was moderate, with the acetone–water extract showing the highest selectivity towards HeLa cells, likely due to its ability to extract both photopigments and phenolics. Co-treatment with doxorubicin enhanced the cytotoxic effects. These findings highlight the potential of Triticum spelta extracts to improve the efficacy of conventional chemotherapeutic agents. spelt grass spelt grass juice spelt water extract potassium phosphorus manganese chromium phenolic compounds phytochemical composition organic farming human malignant cells doxorubicin anticancer drugs cytotoxic effects pira sok pire izvleček pire ekstrakcija kalij fosfor mangan krom fenolne snovi fitokemijska sestava organsko kmetijstvo človeške maligne celice zdravila rak (medicina) citotoksični učinki Proteus anguinus parkelj true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-16 10:47:31 2025-06-16 10:47:37 2025-06-17 04:24:58 0000-00-00 00:00:00 2025 0 0 16 str. iss. 9, art. 4917 Vol. 16 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 633.111 2076-3417 doi.org/10.3390/app15094917 239297539 Spelt_Grass_Juice__Phytochemicals_and_Antiproliferative_Activity.pdf Spelt_Grass_Juice__Phytochemicals_and_Antiproliferative_Activity.pdf 1 E61F8CFA47B404E2EF032A52C1702B7B f7ef62a8452cc2e8fc5df1fb9d4f48bf537cde18f74084a29b9d827f57ba7355 869bf064-4a90-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211690 https://www.mdpi.com/2076-3417/15/9/4917 1 0bb9f4f2-4a8d-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211689 Biotehniška fakulteta 0 0 0