20.500.12556/RUL-169660 Pufrski sistemi in diferencialna spektroskopija Buffer systems and derivative spectroscopy Diplomsko delo obravnava uporabo diferencialne spektroskopije za izboljšanje spektralne analize v sistemih s prekrivajočimi se UV-Vis absorpcijskimi vrhovi. Glavni cilj je bil oceniti, kako lahko ta tehnika pripomore k prepoznavanju in razlikovanju posameznih sestavin v dvokomponentnih zmeseh. Da bi zagotovil kakovostne spektralne podatke, sem najprej optimiziral hitrost snemanja in tehnike glajenja spektralnih podatkov z uporabo didimium stekla, ki je zagotovil ostre, dobro definirane absorpcijske vrhove za ocenjevanje nastavitev spektrometra. Ta postopek je omogočil izbiro optimalnih merilnih parametrov. UV-Vis spektri posameznih snovi – trinatrijevega citrata, HEPES-a, kalijevega tetraoksalata in kalijevega hidrogen ftalata – so bili posneti in analizirani skupaj z ustreznimi dvokomponentnimi zmesmi s citratom. Primerjava prvih in drugih odvodov osnovnih spektrov sicer ni dala dovolj jasnih rezultatov za razlikovanje komponent v mešanicah, vendar se je diferencialna spektroskopija kljub temu izkazala uporabno za izboljšanje spektralne ločljivosti in natančno določanje položajev absorpcijskih vrhov. This diploma thesis explores the application of derivative spectroscopy to improve spectral analysis in systems with overlapping UV-Vis absorbance peaks. The main objective was to assess whether this technique could aid in identifying and differentiating components in binary mixtures. To ensure reliable spectral data, scanning speed and data smoothing methods were first optimized using a didymium glass filter, which provided sharp, well-defined absorption features for evaluating spectrometer settings. This process led to the selection of optimal measurement parameters. UV-Vis spectra of individual substances ‒ trisodium citrate, HEPES, potassium tetraoxalate, and potassium hydrogen phthalate ‒ were recorded and analyzed alongside their corresponding binary mixtures with citrate. Comparisons of the first and second derivatives revealed that, while derivative spectroscopy did not yield sufficiently clear results for distinguishing components within mixtures, it proved valuable for improving spectral resolution and accurately locating peak positions. UV-Vis spektroskopija diferencialna spektroskopija glajenje podatkov dvokomponentne zmesi didimium steklo UV-Vis spectroscopy derivative spectroscopy data smoothing binary mixtures didymium glass filter true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-06-06 12:25:03 2025-06-06 12:25:25 2025-06-12 12:25:15 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 25814 239149571 Fortuna_Jernej_-_Pufrski_sistemi_in_diferencialna_spektroskopija.pdf Fortuna_Jernej_-_Pufrski_sistemi_in_diferencialna_spektroskopija.pdf 1 2036E55D33B27A9B16C69FA2127F8004 fcde05af1c40e573eba116ea17e51719c1726ed5b510092a8303cd7f8c3cf816 3eb9ca20-42bf-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211182 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0