20.500.12556/RUL-169522 DNA methylation panels for the differentiation of lung and gastric adenocarcinomas from other common primary adenocarcinomas Background/Objectives: Gastric and lung adenocarcinomas are among the most common adenocarcinomas worldwide. Our research aimed to validate methylation biomarkers that differentiate gastric and lung adenocarcinomas from hepatocellular carcinoma, cholangiocarcinoma, colorectal carcinoma, pancreatic adenocarcinoma and paired healthy tissues. Methods: The study analyzed 178 formalin-fixed, paraffin-embedded tissue samples, including 14 gastric adenocarcinomas, 15 lung adenocarcinomas, 15 hepatocellular carcinomas, 15 cholangiocarcinomas, 15 colorectal carcinomas, 15 pancreatic adenocarcinomas and their paired healthy tissues. Methylation status was determined experimentally by methylation-sensitive high resolution melting. The diagnostic panels were validated on bioinformatics datasets from The Cancer Genome Atlas and Gene Expression Omnibus, comprising 1981 and 773 samples, respectively. Sensitivity, specificity, diagnostic accuracy and predictive values for each cancer type were calculated for the experimental, Gene Expression Omnibus and The Cancer Genome Atlas datasets. Results: The gastric cancer-specific panel showed a sensitivity of 78.6–83.9%, a specificity of 86.6–94.6% and a diagnostic accuracy of 89.9–96.1% to differentiate between all tumors, and a sensitivity of 78.6–83.9%, a specificity of 89.2–96.4% and a diagnostic accuracy of 88–96.1% to differentiate between all tumors and healthy tissues. The lung adenocarcinoma-specific panel showed a sensitivity of 61.1–93.3%, a specificity of 70.3–90.8% and a diagnostic accuracy of 74.2–90.6% to differentiate between all tumors, and a sensitivity of 61.1–93.3%, a specificity of 77.9–93.4% and a diagnostic accuracy of 79.2% to 93.1% to differentiate between all tumors and healthy tissues. Conclusions: This study demonstrates the potential of using diagnostic methylation panels to differentiate gastric and lung adenocarcinomas from other common adenocarcinomas and paired healthy tissues. methylation biomarkers gastric adenocarcinoma lung adenocarcinoma hepatocellular carcinoma cholangiocarcinoma colorectal carcinoma pancreatic adenocarcinoma adenocarcinoma differentiation DNA methylation biološki označevalci metilacije adenokarcinom želodca adenokarcinom pljuč jetrnocelični karcinom holangiokarcinom kolorektalni karcinom adenokarcinom trebušne slinavke diferenciacija adenokarcinoma metilacija DNA true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-02 08:31:38 2025-06-02 08:31:46 2025-11-10 10:00:52 0000-00-00 00:00:00 2024 0 0 16 str. iss. 23, art. 4000 Vol. 16 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616-006 2072-6694 10.3390/cancers16234000 218915843 RAZ_Draskovic_Tina_2024.pdf RAZ_Draskovic_Tina_2024.pdf 1 B13B58CD521A2667C42B2CDE493EE603 5298f18fbb468e4c8d9b472b18e437ee672e8d4f066b1d1ab96ba3c10a6ab248 8ca46854-3f7f-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210954 https://www.mdpi.com/2072-6694/16/23/4000 1 f1fde42e-3f79-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210952 Medicinska fakulteta 0 0 0