20.500.12556/RUL-169419 Ultrasound-mediated spatial and temporal control of engineered cells in vivo Remote regulation of cells in deep tissue remains a significant challenge. Low-intensity pulsed ultrasound offers promise for in vivo therapies due to its non-invasive nature and precise control. This study uses pulsed ultrasound to control calcium influx in mammalian cells and engineers a therapeutic cellular device responsive to acoustic stimulation in deep tissue without overexpressing calcium channels or gas vesicles. Pulsed ultrasound parameters are established to induce calcium influx in HEK293 cells. Additionally, cells are engineered to express a designed calcium-responsive transcription factor controlling the expression of a selected therapeutic gene, constituting a therapeutic cellular device. The engineered sonogenetic system’s functionality is demonstrated in vivo in mice, where an implanted anti-inflammatory cytokine-producing cellular device effectively alleviates acute colitis, as shown by improved colonic morphology and histopathology. This approach provides a powerful tool for precise, localized control of engineered cells in deep tissue, showcasing its potential for targeted therapeutic delivery. acute inflammation biophysics calcium signalling metabolic engineering transcription biotehnologija akutna vnetja biofizika kalcijeva signalizacija metabolno inženirstvo true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-28 08:36:42 2025-05-28 08:36:42 2025-05-29 04:38:55 0000-00-00 00:00:00 2024 0 0 14 str. art. 7369 Vol. 15 2024 0000-00-00 Zaloznikova Objavljeno apc 0000-00-00 0000-00-00 0000-00-00 60 2041-1723 10.1038/s41467-024-51620-2 206774787 s41467-024-51620-2.pdf s41467-024-51620-2.pdf 1 664F51CBFE59F97F1E114801B82BAFF1 a7f4cbee8c40155c51552c777ecb2cc4ebd14f3c4919e1677048a55f2ea6abad 3ecb6f2c-3b8d-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210752 https://www.nature.com/articles/s41467-024-51620-2 1 d3f69eaf-3b8c-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210751 Medicinska fakulteta Fakulteta za elektrotehniko 0 0 0